3rd World Congress on

Applied Microbiology

Scientific Program

Keynote Session:

Meetings International -  Conference Keynote Speaker Raimondas Jasevicius photo

Raimondas Jasevicius

Vilnius Gediminas Technical University, Lithuania

Title: Numerical simulation of oblique interaction of bacteria

Biography:

Raimondas Jasevicius has completed his PhD from Vilnius Gediminas Technical University (VGTU) and Post-doctoral studies from Vilnius University, Lithuania. He is a senior researcher, Institute of Mechanics and is an Associate Professor, Department of Department of Mechanical and Material Engineering, VGTU. He has built an adhesive dissipative interaction model after years of experience in research with Otto von Guericke University and Berlin Technical University, Germany. He has more than 18 published articles in reputed journals.

Abstract:

Bacteria have a micro-sized diameter, which can give them the ability to adhere to the surface due to the influence of adhesion. In this paper, the attractive-dissipative behaviour of a bacterium with equations for unloading and separation is considered. This amount of energy dissipation can be predicted, for example, from atomic force measurements, using the analysis of the normal force-displacement diagram, which has the character of hysteresis. Here, knowledge of the energy dissipation mechanism is used due to the influence of adhesion from previous studies with ultrafine solids and its application to the cell. The interaction at tangential direction become specific by considering the influence of adhesion. A study of cellular mechanics can help understand the deformation of a biological object or even smaller molecular interaction mechanisms. The specific interaction of bacteria is an important part of the formation of bacterial biofilms, as well as the spread of bacteria in the environment. This theory is suitable for spherical bacteria, while it can be easily applied to colloidal particles. Consideration of the theory of surface change can also be accepted for non-spherical bacteria. The results of a numerical experiment show the ability of bacteria to slip on the surface. Hydrodynamic force is essential for the movement of bacteria and must be considered. In future studies, it is necessary to take into account the interaction of bacteria with biological surfaces, for example, when it moves in a vessel.

Meetings International -  Conference Keynote Speaker Ivana Haluskova Balter photo

Ivana Haluskova Balter

French society of immunology, France

Title: Antiinfectives and microbiota in era of antimicrobial resistance

Biography:

Ivana Haluskova Balter is a french medical professional specialized in infectious diseases, internal medicine, certified in immunology and pediatric, MBA vaccinology and years of clinical practice. She is also certified in health and diplomacy with live multi-country medical field experience in Southeast Asia (India in particular), West/Central/East Europe. She has over 17 years of experience in senior medical lead positions in pharmaceutical research. She is a member of World alliance against antibiotic resistance and French immunology society (SFI) administrative boards and several international academic societies with focus at the innovation of R&D, reflecting immunology and genetic variability, the role of immunologic approach for treatment and diagnostic to tackle the problem of resistance and the role of microbiota to empower host immune response with extensive clinical application. She is also the member of the international advisory group (CHD India) in order to attract attention to the crucial importance of health issues for sustainable economic development and security.

Abstract:

Bacteria, viruses, parasites and fungi that are resistant to drug cause 700,000 death each year. By 2050 superbugs inured to treatments could cause up to 10 million deaths annually and costs the global economy US$100 trillion. AMR resistance is regarded nowadays as a major threat to global public health. The issue is receiving high-level political attention (G7 and G20 in 2017 for first time). The list was drawn up in a bid to guide and promote research and development (R&D) of new antibiotics, as part of WHO’s efforts  for AMR (27th Feb 2017). Antibiotics shape the ecology of the gut microbiota in profound ways, causing lasting changes to developing and mature microbiotas. The application of next-generation sequencing has enabled detailed views of the side effects these drugs have on commensal populations during treatment of infections. The gut microbiota is relatively stable in healthy adults but the composition of the gut microbiota can change rapidly due to various reasons - nutrition related factors, birth mode, use of antibiotics etc. In animal models, treatment with antibiotics revealed behavioural differences and reduced intestinal permeability.  Preclinical findings indicated that antibiotic treatment impact metabolic function as observed in other studies studying microbiota functions. High throughput sequencing reveals the incomplete, short term recovery of infant gut microbiota following parenteral antibiotic treatment with ampicillin and gentamicin. Pediatric dose of penicilin in a mice model, showed significant effects  in terms of social behaviour, social interaction, social avoidance. Experimental mice that were given the probiotic bacteria Lactobacillus rhamnosus  JB-1 in addition to the antibiotics showed fewer of the changes in brain biology and behaviour. The experiment showed not only that changes in gut microbiota could affect the brain, but also that the specific type of bacteria mattered for the end result. Antibiotic use is associated with alteration of the gut microbiome and metabolic activity. Investigations were done in order to study the association between infant antibiotic exposure (aged < 24 months) and childhood obesity or overweight. Antibiotic exposure in infants, aged < 24 months, was associated with a small increase in odds of childhood overweight or obesity in some subgroups of children.  It is known that he first 3 years of life (also termed a ‘window of opportunity’) may represent the most critical period for dietary interventions aimed at gut microbiota modulation for improving child growth and development. In this context, disruption of early-life interactions between host and gut microbiota could have lasting effects by affecting host immunological and metabolic development. A recent study has found that early-life antibiotic exposure in non-obese diabetic mice may accelerate T1D development. Advances in clinical research and development in area of anti-infectives coupled with a renewed interest in the use of probiotics, FMTs (fecal microbiota transplantation) and phage therapy along with thoughtful development of vaccines and monoclonal antibodies represents multiple paths in approach to tackle AMR considering preservation of microbiota.  New medications that protects the gut microbiota (and gut dysbiosis) and preserve antibiotic efficacy are under clinical development. Despite the initial existing preclinical findings, scepticism is warranted when extrapolating findings to human physiology and disease. There is currently limited evidence from epidemiological or high-quality clinical studies to show major effects of the normal gut microbiota or microbiota modulation with dietary changes, prebiotics, probiotics, or antibiotics on gut-brain interactions or on brain function (i.e., affect, cognition) in healthy adult humans or in human disease. From long-term perspective, the research could have serious implications for those living with mental or behavioural problems. In the context of raising antimicrobial resistance, the World Health Organisation has launched the Global Antimicrobial Resistance Surveillance System (GLASS) initiative in order to strengthen bacteriology laboratories in low-and middle income countries for targeted antibiotic resistance surveillance and this might be occasion to gather better understanding and  necessary information. In conclusion, there is a need for extended clinical research triggered by burning issue of anti-infective resistance and  surveillance studies monitoring antibiotic resistance which include infants (early in life microbiota alteration ) would bring useful additional insights. It is an occasion to gather more information on the microbiota’s effects, role of immune and neuroendocrine system and to help in understanding and preventing gut microbiota mediated pathologies in humans and the most appropriate interventions and tools to be used.

Meetings International -  Conference Keynote Speaker Jean Marie Poffe photo

Jean Marie Poffe

Nutrition Cellulaire, Belgium

Title: Medical bacteriology

Biography:

Jean Marie Poffe is a pharmacist since 20 years and completed his pharmacist diploma in 1976. He received a certificate of pharmacist specialization in clinical bacteriology. He has also done specialization in nutrition and micro nutrition and works as a nutrition consultant at Nutrition Cellulaire, Belgium.

Abstract:

It is consisting of the analyte of so many biological liquids and will characterize all the batteries that will be responsible for the diseases. After the identification of the batteries responsible, we make an antibiogram to give the best antibiotic with a good effect on the batteries. We will make a distinction between the open products and close products. A battery that will be isolated from a close product is considered as a pathogen. In the open product, pathogens batteries don’t give any problems (Salmonella Typhi, Shigella, etc.) The opportunist batteries: a very big problem. A person can be healthy carrier, she will not develop the disease BUT she can contaminate other persons.

Oral Session 1:

  • Food Microbiology | Pharmaceutical Microbiology | Clinical Microbiology
Speaker

Chair

Raimondas Jasevicius

Vilnius Gediminas Technical University, Lithuania

Meetings International -  Conference Keynote Speaker Aycan Cinar photo

Aycan Cinar

Bursa Technical University, Turkey

Title: Effect of organic honey, apple and grape vinegar on biofilm forming bacteria

Biography:

Aycan Cinar completed her PhD on food microbiology at Uludag University, Bursa, Turkey. She has involved in Breast Cancer Campaign project as a Post-doctoral reseacher at Brunel University, London, UK. Currently, her role is Assistant Professor at the Department of Food Engineering in Bursa Technical University. Her researches focused on antibacterial, antifungal and anticancer activity of bee products, medicinal plants and natural compounds.

Abstract:

Microorganisms develop a unique set of strategies to overcome the challenges in the environment, such as human beings and all living organisms. One of these strategies is the complex structure called biofilm, which gives bacteria the ability to grow and survive under harsh environmental conditions. The present study investigated the antibacterial and antibiofilm effect of organic honey vinegar (HV), apple vinegar (AV) and grape vinegar (GV) at different concentration. The effect of HV, AV and GV in inhibiting biofilm formation was determined with crystal violet staining assay. Moreover, the effect on the biofilms formed after 48 hours (biofilm inactivation) were determined by different treatment times (30, 60, 120 s). The biofilm removing efficacy of the different treatment times using HV, AV, GV were compared using the BFI (Biofilm Forming Index).

Meetings International -  Conference Keynote Speaker Celia Vargas photo

Celia Vargas

The National University of San Marcos, Peru

Title: Phylogenetic analysis of native Lactobacillus strain isolated from Vaccinium floribumdum kunth using 16S rDNA gene

Biography:

Celia Vargas is pharmaceutical chemist. She has completed her MSc in pharmaceutical biotechnology from University of Bologna, Italy. She is doing a PhD in biological science and engineering from National Agrarian University, Peru. Presently, she is the Professor at Faculty of Pharmacy and Biochemistry from University San Marcos, Peru and member at Centro Latinoamericano de Ensenanza e Investigacion en Bacteriologia Alimentaria, CLEIBA.

Abstract:

The present study investigated berries that grown in a wild way in Peru above 3500 m.a.s.l. and these have become medicinal importance due to its high concentration of antioxidants, and several species of Lactobacillus, which are part of their native flora, could be a valuable tool in order to increase natural benefits in different foodstuff. This study aims to analyze phylogenetics and taxonomy based on 16S rDNA gene allows differentiation at species level. The bioinformatics analysis (BLAST and reconstruction of phylogenetic trees), found the genus Lactobacillus, Leuconostoc and Weissiella. Some strains showed similarity about 100% with Weissella cibaria KACC 11862, Leuconostoc mesenteroids subsp. dextranicum and Leuconostoc mesenteroids subsp. mesenteroids located in the same clade in the phylogenetic tree, which would confirm its phylogenetic approach. The native strains of the genus Lactobacillus showed similarity with L. plantarum, L. paracasei, L. fermentum and L. pentosus, while others native strains of same genus were located in different clades, confirming a probable diversity of species. Due to these results it is suggested to carry out the phylogenetic analysis with more specific genes for their taxonomic location.

Meetings International -  Conference Keynote Speaker Glory I Baysah photo

Glory I Baysah

Adventist University of West Africa, Liberia

Title: Antibiogram of bacterial isolated from well water as sources of drinking water in Margibi County, Liberia

Biography:

Glory I Baysah has completed the PhD degree at the age of 39 years from Babcock University. She is presently the Vice President for Academics. She has also served on many administrative positions and published more than 6 papers, and presently working on different articles.

Abstract:

Water is obtained from harvesting of rain, bodies of water (rivers, ponds) or from ground water (wells, springs and boreholes). Although water can contain unwanted chemicals (from natural sources and agricultural activities), the greatest risk to human health is from faecal contamination of water supplies causing water- borne diseases. Microbial load of borehole water samples from seven areas within Kakata Margibi County Liberia was determined using standard microbiological methods. Susceptibility of the bacteria isolated to commercial antibiotics was also assessed. Results showed that total aerobic plate count ranged from 1.9x10 to 5.4x10² cfu/ml, while total coliform count ranged from 0.6x10 to 1.8x10 cfu/100ml. Faecal coliform count was generally low in most samples and completely absent from sample from one of the study areas. Microbial counts differed significantly (p=0.05) among the various locations studied. Predominant bacteria isolated included Staphylococcus aureus, Enterococcus species, Salmonella species, Citrobacter species, Escherichia coli and bacillus species. The antibiogram carried out using the disc diffusion technique showed that Staphylococcus aureus was most sensitive to streptomycin and least to ciprofloxacin, Enterococcus species was most sensitive to septrin but resistant to many of the antibioties tested.  Escherichia coli were most sensitive to septrin and least to ciprofloxacin. On the contrary, Ciprofloxacin was highly inhibitory to Citrobacter species in the same was as nalidixic acid. Salmonella species were highly sensitive to nalidixic acid and augmentin, while the Bacillus species was resistant to all the antibiotics tested which is of public health concern.

Meetings International -  Conference Keynote Speaker Zlatka Alexieva photo

Zlatka Alexieva

Bulgarian Academy of Sciences, Bulgaria

Title: Temperature effect on the degradation of phenol and catechol by Antarctic strain Lecanicillium sp. AL12

Biography:

Zlatka Alexieva has graduated in the specialty of biochemistry and microbiology from University of Sofia and has completed her PhD from Institute of Molecular Biology, Ukrainian Academy of Sciences at 1985. She has specialized in molecular biology in University of Baltimore, for one year. She was the Head of Division of General Microbiology and Head of Department of Microbial Genetics at the Institute of Microbiology, Bulgarian Academy of Sciences. She has published more than 120 scientific papers published in national and international journals and conference proceedings. The list of citations includes more than 700 citing publications.

Abstract:

The ability of various microorganisms to decompose aromatic contaminants has been actively investigated for many years. However, there is very little available scientific information on Antarctic eukaryotic microorganisms and their industrial potential, although their presence there has long been established. The aim of the present study is to examine the ability of a fungal strain belonging to the genus Lecanicillium and isolated from a soil sample from Antarctica to degrade phenol and catechol in mesophilic conditions (23°C) and low temperature (10°C). The results show that the test strain degrades catechol in a higher concentration (0.3 g/l) than phenol (0.2 g/l) within about 400 hours. It was found that the activity of two key for degradation of phenolic compounds enzymes, such as phenol hydroxylase and catechol 1,2 - dioxygenase differ between the two temperature regimes, as at 10° C were lower. However, we observe that the time required for complete degradation of the two substrates does not differ significantly in the two temperature modes of cultivation. This effect may be due to the relatively low activity of phenol hydroxylase (lower than 0.1 U/mg proteins) with respect to both substrates at both test temperatures, resulting in an equally slow first decomposition step. The highest catechol 1, 2-dioxygenase activity was measured in the degradation of catechol at 23°C - 0.271 U/mg proteins, while at 10°C, it is 108 U/mg protein. These data indicate the ability of the test strain to degrade polyaromatic compounds where the catechol-1, 2-dioxinase enzyme activity is important.

Meetings International -  Conference Keynote Speaker Soria Hamdaoui photo

Soria Hamdaoui

Consultys, France

Title: How to establish growth promotion tests for pharmaceutical culture media

Biography:

Soria Hamdaoui has completed her Master in applied microbiology and biotechnology at the age of 24 years from Paris XI University. She has been the Microbiology and Toxicology Laboratory Manager for 10 years at Intertek Group PLC and developed in vitro testing and  microbiology testing activities in laboratories under ISO / IEC 17025 accreditation and Good Laboratory Practices. It included quality control tests on drugs (oral, topical, oral liquid dose, single use systems, etc), and packaging but also claim support testing for cosmetic formulations and cytotoxicity tests for medical devices. She is now working as a Senior Pharmaceutical Consultant to support and advise pharmaceutical and medical device industries.

Abstract:

The present presentation is about growth promotion test and how it could be implemented in pharmaceutical industry. Culture media are used in most assays in a microbiology quality control laboratory, and if the media does not properly support growth, false negative results may be obtained. The presentation discusses some of the guidance documents and regulatory expectations regarding media growth promotion and provides guidance on establishing a compliant growth promotion test.

Meetings International -  Conference Keynote Speaker Aycan Cinar photo

Aycan Cinar

Bursa Technical University, Turkey

Title: The antimicrobial properties of bee bread

Biography:

Aycan Cinar completed her PhD on food microbiology at Uludag University, Bursa, Turkey. She has involved in Breast Cancer Campaign project as a Post-doctoral Reseacher at Brunel University, London, UK. Currently, her role is Assistant Professor at the Department of Food Engineering in Bursa Technical University. Her researches focused on antibacterial, antifungal and anticancer activity of bee products, medicinal plants and natural compounds.

Abstract:

Bee bread is derived from the transformation of plant pollen by bio-chemical processes caused by the enzymes in the saliva and gastric fluid of the honeybee. The main constituents of the bee bread are carbohydrates (24-34%), proteins (14-37%), crude fibre (1-3%), lipids (6-13%) and lactic acid (2-4%). Minor components are constituted decanoic acid, gamma globulin, nucleic acids, vitamins B and C, pantothenic acid, biopterin, neopterin, acetylcholine, and reproductive hormones. The structure of pollen and bee bread is similar. However, the main difference between bee pollen and bee bread is fermentation process. Bee bread fermentation begins with a wide variety of species, including many fungi, yeast and bacteria derived from collected pollen and bee gut microbiota. The fermentation process follows a succession of the microbial community toward prevailing lactic acid producing bacterial species. Microorganisms, which provide conversion to the bee bread, produce vitamins, enzymes, lipids, substances with antimicrobial effects like bacteriocin molecules, organic acids like fatty and lactic acids. Due to its constituents and formation mechanism, bee bread has showed various degrees of inhibitory effect against the B. subtilis, S. aureus, E. coli and Salmonella. Also, bee bread inhibits the growth of a broad group of microorganisms resistant to antibiotics (Escherichia coli, Staphylococcus aureus, Bacillus cereus and Pseudomonas aeruginosa) and this activity is higher for Gram positive than Gram-negative bacteria.

Meetings International -  Conference Keynote Speaker Zlatka Alexieva photo

Zlatka Alexieva

Bulgarian Academy of Sciences, Bulgaria

Title: GC-MS analysis of LMW PAHs degradation by yeast Trichosporon cutaneum R57

Biography:

Zlatka Alexieva has graduated in the specialty of biochemistry and microbiology from University of Sofia and has completed her PhD from Institute of Molecular Biology, Ukrainian Academy of Sciences at 1985. She has specialized in molecular biology in University of Baltimore, for one year. She has been the Head of Division of General Microbiology and Head of Department of Microbial Genetics at the Institute of Microbiology, Bulgarian Academy of Sciences. She has published more than 120 scientific papers published in national and international journals and conference proceedings. The list of citations includes more than 700 citing publications.

Abstract:

Among the most difficult to degrade chemical pollutants are known polyaromatic substances (PAH). A number of microorganisms capable to varying degrees to transform or degrade PAHs have been described. The yeast strain T. cutaneum R57 is known from previous research as a strain capable of degrading efficiently phenol and a number of derivatives thereof. Our recent studies have focused on its possibility to degrade three low molecular weight (LMW) compounds of this group: naphthalene, anthracene and phenanthrene. The strain was grown at aeration and at 28°C in a culture medium containing each of the test compounds included as the sole carbon source. The following initial concentrations were used: naphthalene - 0.36 g/l; anthracene - 0.39 g/l; and phenanthrene - 0.37 g/l. In the same conditions, the influence of glucose (0.1 g/l) on the degradation process was also tested. By gas chromatography-mass spectrometry (GC-MS) was determined different rates of degradation of the tested compounds. The strain T. cutaneum R57 degrades an average of 51 mgl-1day-1 of naphthalene, 16 mgl-1day-1of anthracene and 10 mgl-1day-1 of phenanthrene. It was found that when using a low concentration of an additional carbon source such as glucose, the rate of degradation is delayed insignificantly, within 3-5%.

Meetings International -  Conference Keynote Speaker Aycan Cinar photo

Aycan Cinar

Bursa Technical University, Turkey

Title: Hazard analysis critical control point (HACCP): Implementation in the frozen food industry

Biography:

Aycan Cinar completed her PhD on food microbiology at Uludag University, Bursa, Turkey. She has involved in Breast Cancer Campaign project as a Post-doctoral Reseacher at Brunel University, London, UK. Currently, her role is Assistant Professor at the Department of Food Engineering in Bursa Technical University. Her researches focused on antibacterial, antifungal and anticancer activity of bee products, medicinal plants and natural compounds.

Abstract:

Freezing process is the best protection methods against microbiological and chemical spoilage of foods. Therefore, frozen food consumption is increasing day by day and every day a new product, a new brand takes place on the shelves. On the other hand, one of the largest foodborne disease outbreaks recorded in the world comes from frozen foods and the number of deaths and poisoning increases each year. Consumption of frozen food contaminated with pathogen microorganisms (PMO) could be more serious for older adults or anyone with a weakened immune system, including pregnant women and newborn children.107 countries received frozen vegetables recalled for Listeria on July 19, 2018 (1). Food recalls are most importantly a public health issue and also they are also significant economic issues and cost of a recall to a Frozen food company is very high, besides to brand damage and lost sales. The Hazard Analysis Critical Control Point (HACCP) is a system to identify, assess and control the hazards related with production, processing, distribution and consumption in order to get safe food(2). The aim of this study was to design a HACCP system for implementing in processing line of frozen Pepper in selected frozen food factory. The following steps were taken for the HACCP system application respectively: HACCP team has been selected; product definitions and usage purposes have been formed, created the flow diagram of the frozen pepper, monitoring system, corrective actions and records created.

Meetings International -  Conference Keynote Speaker Inga Burneikiene photo

Inga Burneikiene

Vilnius University, Lithuania

Title: The analysis of antimicrobial peptides production of lactic acid bacteria

Biography:

Inga Burneikiene is a PhD student in Vilnius University. She works as junior assistant in Department of Microbiology & Biotechnology, Life Sciences Center, Vilnius University.

Abstract:

Microorganisms such as molds, yeast and bacteria can cause a lot of loses in food industry and agricultures. So, researchers are looking for new more effective and environmentally friendly ways to control of growth of bacteria and fungi. Lactic acid bacteria (LAB) and their supernatants are quite promising tools in this area. During the fermentation process, lactic acid bacteria produce variety of anti-microbial agents, such as organic acid, carbon dioxide, ethanol, hydrogen peroxide, diacetyl as well as bacteriocins (John et al. 2007). They contribute to extend the expiration date of foods by using above mentioned anti-microbial agents as natural bio-preservatives and bacteriocins are also a great alternative to common antibiotics to which bacteria gain resistance. In our study we used lactic acid bacteria cultures newly isolated from different sources: sour cow and gout milk, pickled vegetables, bread sourdough. The genetic analysis of LAB isolates allowed to group them into different strains and confirmed that they have bacteriocin-encoding genes in their genomes. 16s rDNA sequencing showed that strains belong to Lactobacillus sp., Lactococcus sp., Pediococcus sp. and Leuconostoc sp. The analysis of an influence of aerobic and anaerobic conditions on the growth and antimicrobial activity against two sensitive strains (Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus epidermidis (ATCC 12228)) of LAB isolates in 37 °C temperature showed that a biomass growth depends on bacteria isolate, not on growth’s conditions, and the highest antimicrobial activity was determined in the stationary growth phase and it correlated with biomass quantity.

Meetings International -  Conference Keynote Speaker Olga Savinova photo

Olga Savinova

Russian Academy of Sciences, Russia

Title: Biocatalytic transformation of the progesterone by the Aspergillus nidulans VKPM F-1069 and by the genetically modified strains obtained on its basis

Biography:

Olga Savinova is a junior researcher at the Laboratory of Molecular Aspects of Biotransformations, A. N. Bach Institute of Biochemistry, Research Center of Biotechnology, RAS. She has participated in various international conferences and has publications in the leading scientific journals. Her scientific interests: involves biocatalytic transformation of steroids by filamentous fungi, heterologous expression of enzymes in ascomycetes, the study of the proteome and secretome of fungi.

Abstract:

The targeted introduction of various functional groups in the steroid molecule determines the diversity of physiological activities of the compound. Hydroxylation of the progesterone (PG, I) has great scientific and practical interest. However, 11-hydroxylation of PG in a single stage with a high degree of regio- and stereo- selectivity is only possible biotechnologically using microorganisms (mainly filamentous fungi) enzyme systems. The present study investigated the ability of the A. nidulans VKPM F-1069 (syn. FGSC A4) and genetically modified strains A.nidulans 031(argB-, pyrG-) (syn. AN031), A.nidulans lacâ„–4 (argB-), and A.nidulans 031/pN8 (pyrG-) to transform PG. It was established that all used strains are capable to transform of PG with the formation of 3 main products: 11α-hydroxy-PG (II), 11α-acetoxy-PG(III) and 6β,11α-dihydroxy-PG(IV), but in different ratio. However, the accumulation of 11α-hydroxy-PG does not occur, since it is further modified into compounds III and IV. 11α-Acetoxy-PG can be converted into compound II by the known methods of hydrolysis. The maximum total relative selectivity of the compounds II and III formation (68.4% mol) was observed during the transformation of PG by the A.nidulans lacâ„–4 strain. The yield of the target products can be increased by optimizing the cultivation conditions of the strain and transformation conditions. The ability of Aspergillus fungi to acetylate 11α-hydroxy-PG has been established by us for the first time. The compounds II и III are of interest not only as precursors in the synthesis of highly active steroid drugs, but also as independent drugs with gestagen and antiandrogenic activity.