Department of Pharmaceutical Analysis, Institute of Pharmacy, Nirma University, Gujarat, India
Dr. Charmy S. Kothari has 18 years of teaching and research experience. Her research areas of interest are analytical and bioanalytical method development and validation, impurity profiling and stability studies as well as Isolation, identification and characterization of marker compounds from plants and formulations. Her research area also includes regulatory guidelines, registration procedures, evaluation and approval procedures of various regulatory agencies worldwide as well as Pharmacovigilance system. She has more than 50 research and review papers published in reputed Indian and International journals.
A rapid and sensitive high-performance liquid chromatography (HPLC) method was developed and validated in human plasma for simultaneous determination of rosuvastatin and candesartan, using atorvastatin as internal standard. Chromatographic separation was achieved on a Waters C18 column (250 × 4.6 mm, 5 µm). A response surface methodology based 3 2 full factorial design was employed to optimize critical chromatographic conditions viz. pH and composition of mobile phase for achieving good resolution between the desired analytes.