International Conference on
Plant Science
Scientific Program
Keynote Session:
Title: Reproductive and Morphological performances of stallions in District of Karo, North Sumatera, Indonesia
Biography:
Yusuf Leonard Henuk is a Professor in the Department of Animal Science, Faculty of Agriculture at the University of Sumatera Utara (USU), Medan, North Sumatera, Indonesia. He participated in the courses of Arabic Language and mainly Poultry Production and Health from 15 January 31 March 2008 organized by the Egyptian International Centre for Agriculture (EICA), Cairo, Egypt. He was a twice Visiting Professor at the Department of Poultry Science, Texas A&M University, College Station, USA (September December 2010& 2017). He was a prolific writer and has published many articles in international journals and mainly in poultry science, e.g. World Poultry Science Journal as well as international scientific meetings such as 3rd World Plant Genomics and Plant Science Congress Keynote Speaker July 15 16, Osaka, Japan; 15th Annual Conference on Crop Science and Agriculture Keynote Speaker 18-19 November 2019, Bali, Indonesia.
Abstract:
The objective of this study was to investigate the reproductive and morphological performances of stallions in the District of Karo, North Sumatera, Indonesia. Body measurements and semen were taken on 12 stallions from four villages in the District of Karo, North Sumatra, Indonesia. There were two villages in Merdeka Sub-district i.e. Jaranguda and Merdeka; and two villages in the Berastagi Sub-district, i.e., Gundaling and Berastagi. Data were entered in Microsoft excel, checked, organized, and processed for further analyses. Means with standard errors (SE) for different traits were subjected to the standard analysis of variance technique. The results showed that the semen quality of stallions performed in the macroscopic and microscopic examination was of good quality with the color of semen was white grey, neutral pH, 74% dilute, 73.3% motility, 13.40 x 107 concentration with their semen abnormalities observed were normal. The morphology characteristics of stallions in the District of Karo showed an appearance with a variation coefficient value below 10% which means the measurement value of horizontal horse morphology is in good condition. The results support horse breeders to cross-breed periodically their stallions with local horses in the District of Karo because their variation coefficient showed good quality semen with uniform value.
Title: Genomic compatibility and inheritance of hexaploid-derived Fusarium head blight resistance genes in durum wheat
Biography:
Xiwen Cai is currently working as a professor at the Department of Plant Sciences at North Dakota State University, North Dakota. His current research is on genomics-enabled chromosome engineering. He has completed his Ph.D. in Crop genetics at Washing State University.
Abstract:
Hexaploid-derived resistance genes exhibit complex inheritance and expression patterns in tetraploid backgrounds. This study aimed to characterize the inheritance patterns and genomic compatibilities of hexaploid-derived Fusarium head blight (FHB) resistance genes in tetraploid durum wheat (Triticum durum Desf.). Evaluation of FHB resistance for F1 hybrids of hexaploid ‘Sumai 3’ crossed with tetraploid and hexaploid wheat indicated that Sumai 3-derived FHB resistance genes exhibit a dominant phenotypic effect seen only in hexaploid hybrids. Alternately, the hexaploid-derived FHB resistance genes from PI 277012 exhibited complete dominance in the crosses with both tetraploid and hexaploid wheat. FHB evaluation of the F1 hybrids of Sumai 3 and PI 277012 crossed with ‘Langdon’ (LDN)– ‘Chinese Spring’ D-genome substitution lines suggested that chromosomes 2B, 3B, 4B, 5B, 6B, 3A, 4A, 6A, and 7A contain genes that suppress the expression of the Sumai 3-derived FHB resistance, whereas chromosomes 4A, 6A, and 6B contain genes required for expression of PI 277012-derived FHB resistance. A wide range of segregation for FHB severity (10–90%) was observed in the F2 generation of Sumai 3 crossed with durum cultivars LDN and ‘Divide’, but the distribution of F3 families derived from the most resistant F2 segregants was skewed towards susceptibility. Similar segregation trends were observed in the crosses of PI 277012 with other durum wheat, whereby FHB resistance became slightly diluted over successive generations. These results suggest tetraploid durum wheat contains the unique alleles at multiple gene loci on different chromosomes that positively and/or negatively regulate the expression of hexaploid-derived FHB resistance genes, which complicate efforts to deploy these genes in durum breeding programs.
Oral Session 1:
- Plant Biotechnology
Title: Effect of U-to-C RNA editing on plant stability
Biography:
Dr. Ruchika is highly motivated scientist from India. She has obtained her bachelor and master’s degree from Delhi University, India. Thereafter she moved to Japan and received doctoral degree in molecular biology from Japan advanced institute of science and technology, JAIST, Japan. For this, she was also affiliated with the Excellent student Award from the president of JAIST. Now, Ruchika has recruited as Assistant professor at Kyushu university, Fukuoka, Japan. She is experienced in RNA editing Techniques and relate molecular biology tools. Currently, she is working for designing an artificial PPRs to target Non-coding RNAs for the clinical application, especially the degenerative diseases or cancers.
Abstract:
Cytidine-to-uridine (C-to-U) RNA editing has been generally observed in land plants; however, reverse (U-to-C) RNA editing is a rare phenomenon. In this study, we investigated the U-to-C RNA editing-related genes in Arabidopsis tissues and the effects on mRNA stability. A previous study showed the extensive occurrence of U-to-C RNA editing in 12-day and 20-dayold Arabidopsis seedlings. Here, we have demonstrated the effects of this “reverse” RNA editing on the mRNA stability for all seven edited genes. We also identified U-to-C RNA editing in the nuclear PPR gene (AT2G19280) in 12-day-old seedlings of Arabidopsis thaliana. The U-to-C RNA editing sites were found in the untranslated region (3’ UTR) of the mature mRNA and may affect its secondary structure. We also examined the correlation between U-to-C RNA editing-related genes and their mRNA abundance. Furthermore, we investigated the effects of U-to-C RNA editing in Arabidopsis using the transcription inhibitor actinomycin D (Act D). The addition of Act D to the cell suspension culture of transgenic Arabidopsis generated by Agrobacterium-mediated transformation showed that single nucleotide base conversion adversely affected the mRNA secondary structure and plant stability.
Title: Establishment of in planta transformation protocol of tomato (Solanum lycopersicum L.) through antiporter gene for improved salinity tolerance
Biography:
Anamika Datta is currently working as a Doctoral Student in Kyushu University, Department of Stem Cell Biology under Faculty of Arts and Science, Graduate School of System Life Sciences, Kyushu University, Japan. She has been receiving scholarship from Japan Science and Technology Agency. She completed her MS in Biotechnology from BRAC University, Dhaka, Bangladesh and worked as a MS fellow under a project titled “Production of tomato tolerant to environmental stresses like salinity”; by BAS-USDA PLAS Endowment fund (Li-03). She developed her skill on plant tissue culture and Agrobacterium-mediated transformation during her working period. She has been worked as faculty member in BRAC University, Dhaka Bangladesh for 3 years. During her research career she has published 2 original research paper, 2 review papers, with 6 conference papers and 2 poster publications in international conferences. Currently she is pursuing her doctoral studies in Neurobiology and Stem Cell related research.
Abstract:
Salinity has been accounted for global production loss of the tasteful and nutritious vegetable “Tomato” lately. In this backdrop, a simple and efficient Agrobacterium mediated in planta transformation protocol has been established, to transform and overexpress Na+/H+ antiporter gene into five Bangladeshi tomato varieties named as BARI tomato 2, BARI tomato 3, BINA tomato 2, BINA tomato 3 and Bahar, to improve their salt tolerance, through optimization of crucial transformation factors like optical density, infection time, co-cultivation period etc. Two vectors were constructed by cloning Na+/H+ antiporter gene from Arabidopsis (pK7WG2_AtNHX1_1.6) and Rice (pK7WG2_OsNHX1_1.6) individually to gateway vector pENTR/D-TOPO and electroporated to Agrobacterium while another vector pBI121 was used as control. During transformation using the three vectors, non- pricked seeds infected under conditions of OD600 1.1-1.4 with 30 min of infection time followed by 24h co-cultivation period were found optimum for achieving more than 90% efficiency for GUS expression and germination percentages. Screening of putatively transformed plantlets were carried out through resistance to Kanamycin 50mg/l in germination medium while Cefotaxime 100mg/l was applied to prevent Agrobacterium overgrowth during co-cultivation. Next, salt stress tolerance has been observed in putatively transformed plants through Leaf Disc Bioassay. Here, tolerance of 100mM salt for 14 days was noted. Meanwhile, no significant morphological changes were observed during acclimatization of putatively transformed plantlets. These findings can be considered as the foundation for further improvement of saline-tolerant tomato development and an essential step toward solving the problem of ensuring global nutritional demands.
