International Conference on

Stem Cell

Scientific Program

Keynote Session:

Meetings International - Conference Keynote Speaker Joel I. Osorio photo

Joel I. Osorio

Regenerage Clinic

Title: A possible neuroregeneration on ASIA-A scale using a Neurostimulator and combinatorial biologics: Bioquantine® and Wharton’s Jelly Mesenchymal Stem Cells as standardized therapy for No Option patients

Biography:

Joel I. Osorio is a MD, CEO and Founder of Biotechnology and Regenerative Medicine at RegenerAge™
(www.regenerageclinic.com). He is  Vice President of International Clinical Development for Bioquark,
Inc. (www.bioquark.com) and Founder and president for the Dr. Jois A.C initiative (www.drjois.com)
Advance Fellow by the American Board of Anti-Aging and Regenerative Medicine (A4M), Visiting
Scholar at University of North Carolina at Chapel Hill (Dermatology).  He is a f ellow in Stem Cell
Medicine by the American Academy of Anti-Aging Medicine.

Abstract:

A complete spinal cord injury (SCI) is the complete sensory and motor loss below the site of spinal
cord injury following acute or chronic destruction, compression, or ischemia of the spinal cord. It constitutes
an inestimable public health issue. The most crucial phase in the pathophysiological process of SCI concerns
the well-known secondary injury, which is the uncontrolled and destructive cascade occurring later with
aberrant molecular signaling, inflammation, vascular changes, and secondary cellular dysfunctions. The use
of our combinatorial biologics based in the combination of a unique polypeptide (Bioquantine®) and
Wharton’s Jelly or Umbilical Cord Mesenchymal Stem Cells (UCMSCs) specifically Wharton’s Jelly
derivate ones represents one of the most important and promising and now safe and tested strategy to
stimulate the neuroregeneration. This combinatorial method attract, among the other sources and types of
stem cells, increased because of their ease of isolation/preservation and their properties. In this review, the
therapeutic role of MSCs is discussed, together with their properties, application, limitations, and future
perspectives. However, despite our deeper understanding of the molecular changes occurring after initial
insult to the spinal cord, the cure for paralysis remains elusive. The current treatment of SCI is limited to
early administration of high dose steroids to mitigate the harmful effect of cord edema that occurs after SCI
and to reduce the cascade of secondary delayed SCI. An array of mesenchymal stem cells (MSCs) from
various sources with novel and promising strategies are being developed to improve function after SCI. In
this review, we briefly discuss the pathophysiology of spinal cord injuries and characteristics and the
potential sources of MSCs that can be used in the treatment of SCI. Our evidence and science based method
(as we previously demonstrated with a patient 2 years ago) is showing a promising alternative on the ASIAA
classification SCI. Added to it, we utilized an improved delivery method (making it ambulatory) for the in
situ application of subdural Whaton’s Jelly MSCs and a unique polypeptide (Bioquantine®). Thereafter we
proceeded with the intrathecal application of an advanced neurostimulator biomedical system obtaining
improved results and faster clinical recovery after only 5 weeks of the started translational protocol.
Keywords: mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, Wharton’s jelly
mesenchymal cells, polypeptide, Bioquantine®, spinal cord injury, neurostimulator system, regenerative
medicine, translational medicine.
Learning Objectives:
- Demonstrate new medical pathways for a possible neuroregeneration.
- Demonstrate the safe use of our combinatorial biologics.
- Demonstrate the use and efficacy of Wharton’s Jelly mesenchymal stem cells.
- Demonstrate the safe and efficacy of and advanced neurostimulator biomedical system.
- Demonstrate the efficacy of therapies of regenerative medicine and interventionist pain and palliative care
management.
Topic description:
Spinal cord injury (SCI) constitutes an inestimable public health issue, with an incidence of 40–80 per
million people per year and continues to be a devastating injury to affected individuals and their families and
exacts an enormous financial, psychological and emotional cost to them and to society. Our innovative
translational medical protocol is based in science and regenerative medicine and can bring another option to
those known as “No Option” patients
The differentiation and regeneration potential of stem cells has been well studied in both preclinical and
clinical investigations. A traumatic spinal cord injury triggers a complex local inflammatory reaction capable
of enhancing repair and exacerbating pathology [Fig. 1]. Spinal cord injury (SCI) constitutes an inestimable
public health issue, with an incidence of 40–80 per million people per year. Wharton’s jelly mesenchymal
stem cells (MSCs) and showed hopeful promise in preclinical research.
 

Oral Session 1:

  • Stem Cell Research
Meetings International - Stem Cell 2024 Conference Keynote Speaker Sudha Banasode photo

Sudha Banasode

Shankarrao Mohite College

Title: Stem Cell Research

Biography:

Dr. Sudha Bansode is a Professor in Zoology at Shankarrao Mohite College, Akluj, and Maharashtra State, India. Recently she has completed her Post-Doctoral Studies at University of California, Riverside, USA. She is a active researcher & passionate teacher in India. Still she has been published above 25 research papers in International Journals & she is interested on Bone Research. Also she has honour of Distinguished Editorial Board Member of several International Journals. She is a own author of Textbook Histological Techniques & Outlines of Physiology. And now she is working on another own reference book Rhythms in Freshwater Crustaceans. She is a University recognized research guide for Ph. D. students in India.

She was a invited Indian Speaker of  OXFORD SYMPOSIUM  on27-29 August, 2014 at Balliol College, Oxford, United Kingdom & CELL SIGNALING & CANCER THERAPY – International Conference at Double Tree, Hilton Chicago on 27-28 September 2017. She was academic visitor of Bangkok- Thailand, Colombo-Sri Lanka, Daira-Daira-Dubai-UAE. Her recent intellectual Interaction is with many International Professional groups.

 

Abstract:

Abstract:

Cell culture has become an indispensable technology in many branches of the life science. It provides the basis for studying the regulation of cell proliferation, differentiation & product form at ion in carefully controlled conditions with processes & analytical tools which are scalable from the level of single cell to in excess of 10kg wet weight of cells. The cell culture has provided the entire human genome, and to dissect the pathway soft intracellular & intercellular signalling which ultimately regulates gene expression. As roller bottle culture is considered the first scale up step for choragic dependent cells from stationary flasks or bottles (Langdonetal2004). This achieved by using all internal surfaces for cell growth, rather than just the bottom of the bottle. The added advantages are that a smaller volume of medium & thus a higher product titre can be achieved the cells are more efficiently oxygenated due to alternative exposure  to medium and gas phase &dynamic systems usually generate higher unit cell densities than stationary systems.

Keywords: Cell culture/Animal tissue culture / stem cell research

 

Meetings International - Stem Cell 2024 Conference Keynote Speaker Vatsala Upadhyay photo

Vatsala Upadhyay

Meerut institute of engineering and technology, Lucknow

Title: Tissue Engineering

Biography:

Vatsala Upadhyay perusing 2nd year B.Tech. in biotechnology from Meerut institute of engineering and technology, PIN-250005 , Meerut ,U.P. affiliated to Dr. A. P. J. Abdul Kalam Technical University, Lucknow.

 

 

Abstract:

Tissue Engineering (TE) is an interdisciplinary field that applies the principles of engineering and life sciences to develop a fully functional human organ in vitro to cope up with the problem of organ scarcity. TE uses living cells (stem cells) and their extracellular components with polymer based biomaterial scaffolds to develop biological tissues for human body repair, such as organ transplants. Stem cells, such as human induced pluripotent stem cells, have driven a paradigm shift in tissue regeneration and the modelling of human disease, and represent an unlimited cell source for tissue regeneration and the study of human disease. The ability to reprogram patient-specific cells holds the promise of an enhanced understanding of disease mechanisms and phenotypic variability.

Scaffold serves numerous functions critical for the success of tissue regeneration. 

It allows cells to attach, grow, proliferate, migrate and differentiate.  Extensive research is going on in the areas of Cornea TE, Bone TE, Dental TC, Skin TE, Cardiovascular TE, Cartilage TE, Liver TE and Urinary bladder TE. Cell sheet engineering, organ printing technology and use of bioreactors provide new insights for TE. On the whole, TE appears to be the new frontier of medicine for its impact on regenerative and reconstructive procedures in humans. Each phase in TE must be understood in an integrated manner from the polymer material properties, to the micro- and macro-architecture of scaffold, to the cell, to the tissue-engineered transplant and finally to the host tissue