Biography

Berta Cecilia RAMIREZ Virologist CNRS since 1996 Institut Cochin CNRS UMR8104 since 2008 22, rue Méchain 75014 Paris France Degrees Habilitation à Diriger des Recherches (HDR) Université Sud - Paris XI, July 2002 Institut des Science du Végétal (ISV), Gif-sur-Yvette, France. «Virus et hôtes : Partenaires en Pathogenèse » Doctorat de Biochimie (PhD) Université Paris Diderot - Paris VII, October 1993 Institut Jacques Monod. (IJM), Paris, France. Biologie Moléculaire des Organismes Eucaryotes. «Exploration de la Biologie Moléculaire du Rice hoja blanca virus » Master of Science University of Maryland, May 1988 Department of Botany, University of Maryland, Maryland, USA. Plant Pathology. «Pathogenesis of infection by Tobacco mosaic virus » Research activity since CR1 CNRS Project Leader of the following scientific studies 2012 - present: «Novel mechanisms of host regulation of HIV infection». Direction of 1 PhD thesis, 2 M2, 3 M1, 1 Licence Pro. Institut Cochin. CNRS UMR 8104, Paris. France. 2008 - 2012: «Assembly and transmission of HIV-1 in T cells». Direction of 1 PhD thesis, 1 M2 of the l’EPHE. Institut Cochin, CNRS UMR 8104, Paris. France. 2009 - 2010: «HIV-1 transmission through viral synapsis». Co-direction of 1 M2, 1 PhD thesis. Collaboration with Professors of the Universidad de Costa Rica (UCR). San Jose. Costa Rica. 2000 - 2005: «Interaction between Faba bean necrotic yellows virus and its plant host». Direction of 1 PhD thesis, 4 DEA, 1Licence and co-direction of 1 PhD thesis. Institut des Sciences du Végétal (ISV), CNRS, Gif-sur-Yvette. France. M2 and PhD thesis direction B. Lacombe, Institut Cochin, Université Paris Descartes - Paris V. M2 (2013) and PhD (2016) director. B. Lacombe is presently applying for a Post-doc position. M. Chazal, Institut Cochin, EPHE. M2 (2012) director. He works presently at the Institut Pasteur. P. Nzounza, Institut Cochin, Université Paris Diderot - Paris VII. PhD (2011) director. P. Nzounza is a Post-doc at Institut Gustave Roussi. J. Valverde, Universidad de Costa Rica. M2 (2009) and PhD (2013) co-director. J. Vega, ISV, Université Paris Sud - Paris XI. PhD (2005) director. J. Vega is Professor at the Universidad Nacional Autónoma de México (UNAM), Guanajuato, Mexico. H. Shimada, ISV and CINVESTAV – IPN, Irapuato, Mexico. PhD (2004) co-director. H. Shimada is Professor at the UNAM, Guanajuato, Mexico. International Scientific Activity Collaborations UCR. Alfonso Garcia. “Nanoparticles as tools in cell Biology” San Jose, Costa Rica. 2016 - present. Ongoing preparation of an exchange program between the Univ. Paris Descartes and the UCR. UCR and Hospital Mexico. Jose A. Bonilla and Maria Paz Lopez. “Viral synapsis in T cells from HIV-1 infected patients”. San Jose, Costa Rica 2009 - 2012. 2 Universidad de Antioquia. Gloria Sanchez. “Viral proteins as cell cycle regulators”. Medellin, Colombia 2002 - 2004. Lectures as Invited Speaker Harvard Medical School. Seminar at the Department of Biological Chemistry. Boston, USA. 2015. Universidad de Antioquia. Seminar at the Virology Department. Medellin, Colombia 2012. EMBO World Lecture Course “Virus – Host: Partners in Pathogenicity”. San Jose, Costa Rica. 2010. Universidad de Costa Rica. Seminar at the Centro de Biología Molecular. San Jose, Costa Rica. 2009. EMBO workshop: RNA virus: replication, evolution and drug design. Vienna, Austria. 2007. XLCV Annual Meeting of the American Phytopathology Society - Caribbean Division”, Cartagena, Colombia. Two keynote lectures. 2006. Universidad de los Andes. “Volver a los Andes II”. Bogotá, Colombia. 2006. Hospital Militar de Colombia. Bogotá, Colombia. 2006. Universidad de Antioquia. Seminar the Cancer and Virology Department. Medellin, Colombia 2004. International Scientific Meeting Organizer 32éme Journée Institut Cochin - JCD Dreyfus “Intracellular immunity and the sensing of pathogens”. (September 2015). Paris. 130 participants from 6 different countries attended the meeting. EMBO World Lecture Course “Virus - Host: Partners in Pathogenicity” (February 2010). UCR, San Jose, Costa Rica. 120 participants of 30 countries attended the course, 23 international speakers, 30 scholarships were given for Ph.D. students to travel to Costa Rica. http://cwp.embo.org/wlc10-01/index.html. Editorship Journal of General Virology, Antimicrobial Agents and Chemotherapy, Archives of Virology, Emerging Microbes & Infections (Nature). Expertise for granting institutions NSF (USA), SENACYT (Panama). Scientific communication at international meetings (2016) PhD Students and I attend at least one meeting per year where we present our results. During the year 2016 we attended 4 international meetings. I gave one oral presentation at the meeting “HIV and SIV Persistence, Remission and Cure” at the Institut Pasteur. Paris. France. I was co-author of 3 of PhD Students’ posters: 2 at “The 40th annual meeting on Retroviruses. Cold Spring Harbor Laboratory”, and one at “The Dendritic Cells meeting”. Shanghai. Chine. Publications Lacombe, B. and Ramírez, B.C., (2017) La spironolactone : somnifère du VIH dans les lymphocytes T ? Médecine/Sciences. 10(33), 846-848. Lacombe, B., Morel, M., Margottin-Goguet F. and Ramírez, B.C., (2016) Specific Inhibition of HIV Infection by the Action of Spironolactone in T Cells. Journal of Virology. Dec. 90, 10972-10980. Lahouassa, H., Blondot, M.L, Chauveau, L., Chougui, G., Morel M., Leduc M., Guillonneau F., Ramírez, B.C., Schwartz, O. and Margottin-Goguet F. (2016) HIV-1 Vpr degrades the HLTF DNA translocase in T cells and macrophages. PNAS. May 10;113(19):5311-6. doi: 10.1073/pnas.1600485113. Dragin, L., Munir-Matloob, S., Froehlich, J., Morel, M., Sourisce, A., Lahouassa, H., Bailly, K., Mangeney, M., Ramírez, B.C., Margottin-Goguet F. (2015) Evidence that HIV-1 restriction factor SAMHD1 facilitates differentiation of myeloid THP-1 cells. Virology Journal. Nov 25; 12(1):201. doi: 10.1186/s12985-015-0425-y. 3 Chazal, M., Nzounza, P., Pique, C. and Ramírez, B.C., (2014). Loss of infectivity of HIV-1 particles produced by mobile lymphocytes. PloS One. Oct 8; 9(10):e109601. Dragin, L., Nguyen, L.A., Lahouassa, H., Sourisce, A., Kim, B, Ramírez, B.C., Margottin-Goguet, F. (2013). Interferon block to HIV-1 transduction in macrophages despite SAMHD1 degradation and high deoxynucleoside triphosphates supply. Retrovirology. Mar 11;10:30. Maudet, C., Sourisce, A., Dragin, L., Lahouassa, H., Rain, J.C., Bouaziz, S., Ramírez BC, Margottin-Goguet F. (2013). HIV-1 Vpr induces the degradation of ZIP and sZIP, adaptors of the NuRD chromatin remodeling complex, by hijacking DCAF1/VprBP. PLoS One. Oct 8;8(10). Nzounza, P., Chazal, M., Guedj, C., Schmitt, A., Masse,J.M., Randriamampita, C., Pique, C. and Ramírez, B.C., (2012). The scaffolding protein Dlg1 is a negative regulator of cell-free virus infectivity but not of cell-to-cell HIV-1 transmission in T cells. PloS One. 7(1):e30130. Perugi, F., Muriaux, D., Ramírez, B.C, Chabani, S., Decroly, E., Darlix, J.L., Blot, V. and Pique, C. (2009). Human Discs Large is a new negative regulator of HIV-1 infectivity. Mol. Biol. Cell. 20, 498-508. Komarova A., Haenni, A.L. & Ramírez, B.C (2009). Viruses versus host cell translation : love and hate stories. In: Advances in virus Research. Elsevier. 73: 99-170. Ramírez, B.C (2008). Tenuivirus. In : Encyclopedia of Virology. 5 vols. (B.W.J. Mahy and M.H.V. Van Regenmortel, Editors), pp. 24-27, Oxford: Elsevier. Ramírez, B.C, Simon-Loriere E., Galetto R. & Negroni M. (2008). Implications of recombination for HIV diversity. Virus Research. 134,64-73. Lageix, S., Catrice, O., Deragon, J.M., Gronenborn, B., Pélissier, T. & Ramírez, B.C. (2007). The nanovirus-encoded Clink protein affects plant cell cycle regulation through interaction with the retinoblastoma-related protein. Journal of Virology. 81, 4177-4185. Vega-Arreguín J.C., Gronenborn B. & Ramírez B.C. (2007). Arabidopsis thaliana is a host of the legume nanovirus Faba bean necrotic yellows virus. Virus Research. 128,81-87. Timchenko, T., Katul, L., Aronson, M. Vega-Arreguin, J.C., Ramírez, B.C, Vetten, H. J. & Gronenborn, B. (2006). Infectivity of nanovirus DNAs: Induction of disease by cloned genome components of Faba bean necrotic yellows virus. Journal of General Virology. 87, 1735-1743. Vega-Arreguin, J.C., Timchenko, T., Gronenborn, B. & Ramírez, B.C. (2005). A functional histidinetagged replication initiator protein: implications for the study of single-stranded DNA virus replication in planta. Journal of Virology. 79, 8422-8430. Charpenteau, M., Jaworski, K., Ramírez, B.C., Tretyn, A., Ranjeva, R. & Ranty, B. (2004). A receptor-like kinase from Arabidopsis thaliana is a calmodulin-binding protein. Biochemical Journal. 379, 841-848. Haenni, A.L. & Ramírez, B.C. (2004). The expression of plant virus proteins: In Viruses and virus diseases of Poaceae . (H. Lapierre and P. Signoret Editors), pp 69-82, INRA Press. Perruc, E., Charpenteau, M., Ramírez, B.C., Jauneau, A., Galaud, J.P., Ranjeva, R. & Ranty, B. (2004). A novel calmodulin-binding protein functions as a negative regulator of osmotic stress tolerance in Arabidopsis thaliana seedlings. The Plant Journal. 38, 410-420. Ramírez, B.C. & Haenni, A.L. (2004). Tenuivirus. In Viruses and virus diseases of Poaceae . (H. Lapierre and P. Signoret Editors), pp 344-348, INRA Press. Ramírez, B.C., Bouloy M. & Haenni, A.L. (2002). The award of the « Interbrew-Baillet Latour de la Santé – 2002 » prize to Robert Krug for outstanding contributions to influenza virus research. Archives in Virology. 147, 1657-1661. Li, M.L., Ramírez, B.C., & Krug, R.M. (1998). RNA-dependent activation of primer RNA production by influenza virus polymerase: different regions of the same protein subunit constitute the two required RNA-binding sites. EMBO Journal. 17, 5844-5852. Nguyen, M., Ramírez, B.C., Golbach, R., & Haenni, A.L. (1997). Characterization of the in vitro activity of the RNA-dependent RNA polymerase associated with the ribonucleoproteins of rice hoja blanca tenuivirus. Journal of Virology. 71, 2621-2627. de Miranda, J.R., Ramírez, B.C., Munoz, M., Lozano, I., Wu, R., Haenni, A.L. Espinoza, A.M. & Calvert, L.A. (1997). Comparison of Colombian and Costa Rican strains of rice hoja blanca tenuivirus. Virus Genes. 15, 191-193. Calvert, L.A., Cuervo, M.I., Ospina, M.D., Fauquet, C.M. & Ramírez, B.C. (1996). Characterization of cassava common mosaic virus and a defective RNA species. Journal of General Virology. 77, 525-530. Ramírez, B.C., Barbier, P., Séron, K., Haenni, A.-L. & Bernardi, F. (1994). Molecular mechanisms of point mutations in RNA viruses. In: Molecular Basis of Viral Evolution. (A. Gibbs and C. H. Calisher, Editors), pp. 105-118, Cambridge University Press. Ramírez, B.C., Garcin, D., Kolakofsky, D. & Haenni, A.L. (1994). Capped non-viral sequences at the 5' ends of the mRNA of rice hoja blanca virus ambisense RNA4. Journal of Virology. 69, 1951-1954. Ramírez, B.C. & Haenni, A.L. (1994). The molecular biology of tenuiviruses - a remarkable group of plant viruses. Journal of General Virology. 75, 467-475. 4 Ramírez, B.C., Lozano, I., Constantino, L.M., Haenni, A.L. & Calvert, L.A. (1993). Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4. Journal of General Virology. 74, 2463- 2468. Ramírez, B.C., Macaya, G., Calvert, L.A. & Haenni, A.-L. (1992). Rice hoja blanca virus genome characterization and expression in vitro. Journal of General Virology. 73, 1457-1464. Morales, F., Niessen, A., Ramírez, B.C. & Castaño, M. (1990). Isolation and partial characterization of a geminivirus causing bean dwarf mosaic. Phytopathology. 80, 96-101. Rodriguez-Vargas, A.M., Fajardo, J.E. & Ramírez, B.C. (1984). Phylogeny of transfer RNA. Origins of life. 14, 547-555.

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Research Interest

Virology,HIV,Microbiology