5th International Summit on

Cancer Research and Diagnosis

Paris, France   March 02-03, 2020

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Scientific Program

Keynote Session:

Meetings International -  Conference Keynote Speaker Mohammed Yahya Alomar photo

Mohammed Yahya Alomar

King Abdulaziz University, Saudi Arabia

Title: Effect of Basil Leaves Extract on Liver Fibrosis Induced by Thioacetamide in Male Rats

Biography:

Mohammed Yahya Alomar, He is working on this topic Effect of Basil Leaves Extract on Liver Fibrosis Induced by Thioacetamide in Male Rats 

Abstract:

Background and Objective: Liver fibrosis is one of the most common chronic diseases worldwide. The current medical therapy of hepatic disorders is ineffective and till now there is no any therapy has successfully prevented the liver diseases and disorders. The present study aimed to investigate the effect of basil leaves extract on liver fibrosis induced by thioacetamide (TAA) in male rats. Materials and Methods: About 40 rats were randomly divided into four experimental groups. Rats of group 1 were served as controls. Rats of group 2 were given 300 mg kgG1 body weight of TAA by intraperitoneal injection, twice weekly. Rats of group 3 were orally supplemented with basil leaves extract at a dose of 300 mg kgG1 body weight/day. Moreover, they were intraperitoneally injected with TAA at the same dose given to group 2. Rats of group 4 were orally supplemented with basil leaves extract at same dose given to group 3. After 6 weeks of treatment, the blood samples and liver tissues were subjected to biochemical and histopathological evaluations. Results: Group 2 showed significantly increases of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase and total bilirubin. Significant decreases in the levels of liver glutathione and superoxide dismutase were observed in rats treated with TAA and basil leaves extract plus TAA. Noticeably decrease of liver catalase was observed in TAA treated rats. Malondialdehyde levels in liver were significantly increased in TAA and basil leaves extract plus TAA treated rats compared with control rats. In rats exposed to only TAA, liver sections showed an abnormal morphology characterized by noticeable fibrosis with extracellular matrix collagen contents and damage of liver cells structure. Administration of basil leaves extract to rats exposed to TAA led to inhibition of biochemical and histopathological alterations. Conclusion: These results confirmed that the protective role of basil leaves extract attributed to its antioxidant effects. Additionally, the obtained results clarify that the basil leaves extract is a potential protective natural therapy against liver fibrosis induced by exposure to TAA.
Key words: Liver fibrosis, liver diseases, basil leaves, thioacetamide, Ocimum basilicum, hepatic disorders, liver cells structure
Meetings International -  Conference Keynote Speaker Melanie Di Benedetto photo

Melanie Di Benedetto

Universite Paris Diderot, France

Title: High expression of apoptosis protein (Api-5) in chemoresistant triple-negative breast cancers: an innovative target

Biography:

Melanie Di Benedetto has completed his PhD at the age of 27 years from Paris13 University and several Postdoctoral Studies from School of Medicine, Paris 13 and Paris 7 University. She is associated professor in Inserm U942 Laboratory. She is the Director of a biology department at technical institute of Paris13 University. She has published more than 30 papers in reputed journals  

Abstract:

Anti-apoptotic protein-5 (API-5) is a survival protein interacting with the protein acinus, preventing its cleavage by caspase-3 and thus inhibiting apoptosis. We studied the effect of targeting API-5 in chemoresistant triple negative breast cancers (TNBCs), to reverse chemoresistance. 78 TNBC biopsies from patients with different responses to chemotherapy were analysed for API-5 expression before any treatment. Further studies on API-5 expression and inhibition were performed on patient-derived TNBC xenografts, one highly sensitive to chemotherapies (XBC-S) and the other resistant to most tested drugs (XBC-R). In situ assessments of necrosis, cell proliferation, angiogenesis, and  apoptosis  in  response  to  anti-API-5  peptide  were  performed  on  the  TNBC  xenografts. Clinical  analyses  of  the  78  TNBC  biopsies  revealed  that  API-5  was  more  markedly expressed in endothelial cells before any treatment among patients with chemoresistant TNBC, and this was associated with greater micro-vessel density. A transcriptomic analysis of xenografted tumors showed an involvement of anti-apoptotic genes in the XBC-R model, and API-5 expression was higher in XBC-R endothelial cells. API-5expression was also correlated with hypoxic stress conditions both in vitro and in vivo. 28 days of anti-API-5 peptide efficiently inhibited the XBC-R xenograft via caspase-3 apoptosis. This inhibition was associated with major inhibition of angiogenesis associated with necrosis and apoptosis. API-5 protein could be a valid therapeutic target in chemoresistant metastatic TNBC

Meetings International -  Conference Keynote Speaker Naeem-Ul-Haq Khan photo

Naeem-Ul-Haq Khan

University of Melbourne ,Pakistan

Title: Automated preparation of [18F]-MG11, using [18F] F-Py-TFP, [18F] SFB and [18F] NFP radiosynthons, through iPHASE FlexLab radiosynthesis platform, for PET imaging of cancer

Biography:

Naeem-Ul-Haq Khan has recently submitted his PhD dissertation at Govt. College University, Faisalabad-Pakistan and performed his research work at University of Melbourne, and Peter MacCallum Cancer Centre, Melbourne, Australia. He worked as a research associate at Pakistan Institute of Nuclear Science and Technology, Islamabad-Pakistan. He has published more than 6 papers in good impacted journals and presented his research work in more than 12 national and international conferences.

Abstract:

For diagnosis of malignancies and other disease conditions, [18F] FDG and to lesser extent the other small molecules have become important clinical radio tracers. In USP, only three [18F]-radiopharmaceuticals [18F] FDG, [18F] fluorodopa and [18F] NaF have been listed for PET imaging. [18F]-labeled peptides have also been developed as promising target specific imaging agents. In this study, cold standard FPro-MG11, FBen-MG11 and FPy-MG11 have been synthesized using solid phase peptide synthesis technique. The three [18F]-radiosynthons, [18F] F-Py-TFP, [18F] SFB and [18F] NFP  have been synthesized through TRACER Lab FX-FN module to radiolabel the model peptide MG11 through its N-terminus and investigate the biological effect of resulting radiotracers. The iPHASE FlexLab module was used to radiolabel MG11 peptide by using these radiosynthons [18F] F-Py-TFP, [18F] SFB and [18F] NFP. Automated peptide radiolabeling experiments for [18F] FNic-MG11 synthesis provided more radiolabeling yield and specific activity than for other two analogs [18F] FB-MG11 and [18F] FP-MG11. Further, all these [18F]-prosthetic groups radiolabeled the peptide in site selective manner. Scintigraphy results provides T/NT ratio 7.02 ± 0.98 which is more than other two and found consistent with biodistribution data. Radioactive experiments like scintigraphy, biodistribution, blocking studies, internalization study, autoradiography and H & E staining study and non-radioactive experiments like ligand binding study, hydrophilicity study demonstrated that all the three analogues of MG11 peptide reacted in a target specific manner and proved suitable for PET imaging of CCK-2 receptor positive cancer.

 

Oral Session 1:

  • Oral Session 1
Meetings International - Cancer 2021 Conference Keynote Speaker Muhammad Usman photo

Muhammad Usman

Former Director General of Agricultural Research System, Pakistan

Title: Cancer Research and Diagnosis is the major tool for the development of health, basic need of daily life, as well as to reduce different types of cancer diseases in the world particularly in South Asia.

Biography:

Muhammad Usman, Former Director General of Agricultural Research System, Government of Pakistan who retired from service after a spotless career of about 32 years with senior level experience on research and development of integrated agricultural production, industries, Agriculture & Horticulture and bioenergy on a sustainable way.

Abstract:

The aims of presentation consist of cancer research, diagnoses, different types of cancer were studied and reported that Cancer Research and Diagnosis is the major tool for the development of health, basic need of daily life, as well as to reduce different types of cancer diseases in the world particularly in South Asia.    Cancer is a group of more than 100 different diseases. It can develop almost anywhere in the body. The term oncology literally means a branch of science that deals with tumors and cancers. The word consist of “onco” means bulk, mass, or tumor while “-logy” means study. In a simple words, oncology is the study of cancer and its treatments in medical science. The most important oncology diagnostic remains the clinical history of the patient. Common symptoms that point towards cancer include fatigue, weight loss, unexplained anemia, fever of unknown origin etc. Oncology depends on diagnostic tools like biopsy or removal of bits of the tumors tissue and examining it under the microscope. Other diagnostic tools include endoscopy for the gastrointestinal tract, imaging studies like X-rays, CT scanning, MRI scanning, ultrasound and other radiological techniques, Scintigraphy, Single Photon Emission Computed Tomography, Positron emission tomography and nuclear medicine techniques etc. The oncologist’s role including. Explaining the cancer diagnosis and stage to the patient. Discussing all relevant treatment options and the oncologist’s recommendations. Delivering high-quality, compassionate care. Helping the patient manage cancer-related pain and other symptoms or treatment side effects. The study reported that the total countries available in the world are 225, consist of (Developed countries = 49, developing countries = 150, observer state = 4, state without partial recognition = 8, unrecognized state = 14). Similarly, South Asia comprises the countries of Pakistan, Bangladesh, India, Bhutan, Maldives, Nepal and Sri Lanka.  In the light of above study, it is proposed that Cancer Research and Diagnosis should be commercialized  for the development of health, basic need of daily life, create employment, generate income, stronger economy, reducing financial crises, global Poverty and hunger in the developing countries of the world particularly in south Asia.

 

Meetings International - Cancer 2021 Conference Keynote Speaker K. M. Yacob photo

K. M. Yacob

Marma Heatth Centre, India

Title:  The pyrexia temperature never damage the cells of brain or harm the body.

Biography:

K. M. Yacob a practicing physician in the field of healthcare in the state of Kerala in India for the last 30 years and very much interested in basic research. My interest is spread across the fever , inflammation and  back pain,. I am a writer. I already printed and published nine books in these subjects. I wrote hundreds of articles in various magazines. After scientific studies we have developed 8000 affirmative cross checking questions. It  can explain all queries related with fever

 

Abstract:

All treatments for fever are based on the belief  that  fits is the result of  41 degree Celsius temperature and  it  damages cells of  brain and body. At the same time there is no evidence based   tests or concrete  diagnosing  methods to the  belief  that  fits and brain damage  is the result of  pyrexia .Necessary ingredients to destroy brain cells  and fits cannot be seen  in fever.In pyrexia or absence of fever  a fainted  patient fell on the floor with unconscious state and destroy cells of brain, and necessary ingredients to  become conscious are same. When disease increases essential blood circulation and energy level also decreases. The vertical height between heart and brain is more than one feet. When the disease becomes severe, ability to pump the  blood to the brain decreases. As a result of this   brain cells are damaged. so the patient might be paralyzed or may even die.In pyrexia or absence of fever,  when blood flow to the brain decreases and fits are formed. There is no other  way than  this  to increase  blood circulation  to the brain.It is  a sensible and discreet  action of brain to protect the  life or organ.

 

Meetings International - Cancer 2021 Conference Keynote Speaker Nina Kastelic photo

Nina Kastelic

University Medical Center, Slovenia

Title: Oncology patient at primary level – learning with simulations in healthcare

Biography:

Nina Kastelic is currently working at the University Medical Center, Slovenia. She is working on the research of Oncology patient at primary level – learning with simulations in healthcare.

Abstract:

Simulations in healthcare are a great way for all healthcare workers to train their skills in a safe environment. Participants get experience in managing difficult interventions with simulations that do not happen regularly in clinical environment. Because of this we have prepared a training program in CHC Ljubljana, Simulation Centre. The program is called Oncology patient at primary level – learning with simulations in healthcare. We think that it is very important for the healthcare teams in primary healthcare level to regularly train in field of oncology care. With training they raise their skill level and reduce stress so they have more confidence in their field of expertise. This also prevents unwanted mistakes in clinical environment and thus raises safety level of our patients.
Oncology patient at primary level – learning with simulations in healthcare is an interactive workshop with a lot of practice work which is done for maximum of 12 trainees. Training is being managed by three instructors who use a method of Learning with simulations in healthcare (theory, skills, simulations, debriefing). This way of education has a lot of benefits especially regarding equipment: simulator of a oncology patient that responds as a real person, simulator of a cannula with blood reflux … We rate this program as a very successful because our trainees have rated it with maximum score in 98% and their knowledge results were excellent (intake knowledge was 40%, outtake knowledge was 85%).
Meetings International - Cancer 2021 Conference Keynote Speaker Ghulam Yaseen photo

Ghulam Yaseen

Saher Welfare Foundation, Pakistan

Title: A Case Report of Acute Myeloid Leukemia cured by Homeopathic Approach

Biography:

Ghulam Yaseen has completed his medical graduation (BHMS) from University of Peshawar. He is the President of a non-government organization, Saher Welfare Foundation that treats blood disorder. He is treating Thalassemia, Hemophilia and all types of blood cancers through Homeopathic medicines from 2000. He has presented several cases, cured by Homeopathy in many national and international conferences.         

Abstract:

Acute Myeloid Leukemia(AML) starts in the inner parts of the bone marrow- spongy material in the bones that produce blood cell. It spreads into the other parts of the body involving lymph nodes, liver & central nervous system.symptoms may include easily bleeding and bruising, fatigued, recurrent infections. A pre-diagnosed case of acute myeloid leukemia preented in Homeopathic opd  with excessive bleeding from gums. His MRI showed multiple lobulated extra-conal masses with enhancing thickening, along with both Zygomatic bones & base of the skull. He was admitted  in another hospital for chemotherapy. He took 3 complete sessions of chemotherapy. But bleeding from his gums was persistent. With the passage of time & treatment, he got worse in his disease level with no positive changes in health status. His eyesight was declared dead. At the end, after a year of treatment, they refused for further treatment. This case shifted to homeopathy. He presented in Homeopathic opd in miserable condition.He wasn’t able to walk. Individualized Homeopathic treatment plan started and  his condition improved  gradually. After treatment of 6 months he was completely fine, without any sign and symptoms of acute myeloid leukemia and bad effects of chemotherapy.

 

Meetings International - Cancer 2021 Conference Keynote Speaker Christin Hendriyani Bonnu photo

Christin Hendriyani Bonnu

Universitas Gadjah Mada, Indonesia

Title: Microrna’s signature and it’s role in carcinogenesis for biomaker of prostate cancer

Biography:

Christin Hendriyani Bonnu has completed his bachelor at the age of 22 years from Universitas Nusa Cendana and now studying at Universitas Gadjah Mada in Biotechnology department. This is the first time for her attending an international conference and she has no intenational publication before. 

Abstract:

The usual diagnostic method to diagnostic Prostate cancer is the PSA (prostate specific antigen) method. However this method is considered to be less effective because the interpretation of the value in cancer patients can be the same as in BPH (Benign Prostate Hypertrophy) patients or other prostate infections. One alternative diagnostic method that can be combined with this usual method to reach best result is finding the molecular signature of this cancer. The aim of this study is to determine microRNAs that can use as biomarkers of prostate cancer. The method used in this study  is microRNA expression analysis in two prostate cancer tissue samples and two BPH tissue samples using Nanostring nCounter ®, validation by microarray database and molecular docking. The result shows that there are 7 miRNAs with p values ​​close to 1. There are hsa-mir-98-5p, hsa-let-7a-5p, hsa-mir-106b-5p, hsa-mir-1-3p which are upregulated and  hsa-mir- 25-3p, hsa-mir-205-5p, hsa-mir-152-3p which are  downregulated in prostate cancer. Microarray database analysis proves the dysregulated microRNAs in prostate cancer are  hsa-mir-25-3p, hsa-mir-106b-5p, hsa-let-7a-5p and hsa-mir-98-5p. The docking result shows that hsa-mir-25-3p has stronger interaction with E2F1 than hsa-mir-106b-5p. The last analysis from Kaplen Meier also shows that all these miRNAs have influence on patient’s survival rate, although it is unsignificantly. Our findings revealed that all these miRNAs have potential to be novel biomarker for prostate cancer patients, especially hsa-mir-25-3p and hsa-mir-106b-5p.

 

Meetings International - Cancer 2021 Conference Keynote Speaker Cindy Yeoh photo

Cindy Yeoh

Memorial Sloan Kettering Cancer Center,USA

Title: Anesthetic management of a patient with Cancer and Hyperhomocysteinemia

Biography:


Cindy Yeoh, workng at Anesthetic management of a patient with Cancer and Hyperhomocysteinemia

Abstract:

A 57 year-old female with a history of uterine and breast cancer s/p chemoradiation presented for cervical LEEP/cone biopsy/D&C. Her medical history was complicated by elevated LFTs with recent hyperhomocysteinemia (> 50micromol/l, normal 4-15). She was seen by a hematologist prior to surgery, and it was concluded that elevated homocysteine levels were due to cancer therapy and alcohol consumption. The procedure was performed under monitored sedation, with 2mg of Midazolam, 50mcg of Fentanyl, and a bolus of 70mg of Propofol followed by a steady infusion of 150mcg/kg/min.
Discussion: Causes of hyperhomocysteinemia include genetic predisposition, acquired deficiencies (folate, B6, B12), malignancies, and renal disease. Elevated homocysteine levels result in thromboembolic complications by causing endothelial dysfunction, increasing procoagulant activity, and decreasing antithrombotic effect.
Challenges of patients with hyperhomocysteinemia undergoing anesthesia are related mainly to the procoagulant state and efforts should be focused on thromboprophylaxis and maintenance of hemodynamics and euvolemia. Nitrous oxide should be avoided as it inhibits methionine synthase and can further increase homocysteine levels. Patients with co-morbidities that include coronary artery disease, peripheral vascular disease, and cerebrovascular disease are at increased risk for peri-operative thrombotic events. This risk is amplified for high-risk procedures under general anesthesia.
Conclusion: In this case, the patient presented for a low-risk procedure. She did not have a history of coronary or cerebrovascular disease, but had risk factors (surgery, age>50yrs, malignancy, cancer therapy) in addition to a hypercoagulable state (due to elevated homocysteine levels) that posed increased peri-operative risk for thrombotic events such as deep venous thrombosis and pulmonary embolus. The decision was made to proceed with monitored sedation over general anesthesia so as to avoid fluctuations in hemodynamics and decrease the risk of venous stasis. The procedure took approximately 45 minutes and the patient recovered uneventfully and was discharged home the same day.
References:
Cascella M. Multidisciplinary approach and anesthetic management of a surgical cancer patient with methyl tetrahydrofolate reductase deficiency: a case report and review of the literature J Med Case Rep 2015 Sep (9) 218 Aggarwai S. Hyperhomocysteinemia: Anesthetic concerns Saudi J Anaesth 2014 8(1)143
Badner NH Preoperative oral B vitamins prevent nitrous oxide-induced post-operative plasma homocysteine increases Anesth Analg 2001;93:1507-10
Meetings International - Cancer 2021 Conference Keynote Speaker Mohammad Fazil photo

Mohammad Fazil

Hakim Ajmal Khan Institute for Literary & Historical Research in Unani Medicine, India

Title: Evaluation of anti-cancer and anti-oxidant activity of Cuscuta reflexa Roxb. extracts on human lung and breast cancer cell lines

Biography:

Mohammad Fazil is current research Evaluation of anti-cancer and anti-oxidant activity of Cuscuta reflexa Roxb. extracts on human lung and breast cancer cell lines 

Abstract:

 The present century has seen an upsurge in cancer morbidity and mortality like never before. The word cancer conjures up the image of a silent killer, a disease that puts significant emotional and financial strain on the patient and the family. Chemotherapy has shown promise in fighting cancer, but is wrought with side effects such as nausea, vomiting, oral ulcers, diarrhoea, hepatotoxicity, myelosuppression and immunosuppression. In Unani medicine, there are many plants having bioactive compounds, which are reported to have anti-cancer activity. Cuscuta reflexa Roxb., known as aftimoon in Unani medicine, is a parasitic plant widely found in temperate and tropical regions of India. Aftimoon is one the constituents of Equigard used in Chinese medicine which has been recommended by New York Medical College for treatment of cancer. Methods: The plant material was authenticated through Indian Agriculture Research Institute, Delhi, India. The extracts of Cuscuta reflexa Roxb. were obtained to measure the anticancer activity with H-1299 and MCF-7 cancer cell lines. Soxhlet extraction was utilized for stem and seeds. The anticancer activity of fractions of each extract obtained by using Flash chromatography was also checked. Besides, the antioxidant activity of each fraction was also checked. DNA binding study supported the results obtained during whole process. The cellular death was detected utilizing ELISA. Observations: The results indicated that extracts of Cuscuta reflexa Roxb. exhibited strong anticancer activities as compared to the fractions of each extract. Cuscuta reflexa Roxb. extracts indicated noteworthy cytotoxicity against human H-1299 and (lung cancer) MCF-7 cancer cells (breast cancer). The best results on lung cancer cell lines were obtained with ethyl acetate extract (IC50=74.48 to 902 μg/mL); while the acetone extract had the best activity on breast cancer cell lines (IC50=51.59 to 240 μg/mL). Both extracts had strongest anti DPPH radical activities as compared to other extracts.  It was concluded that the ethyl acetate and acetone extract of this plant may be used to prepare the Unani medicine for treating lung and breast cancers without any toxicity.