5th International Conference on

Public Health and Nutrition

Vancouver , Canada   October 16-17, 2019

Scientific Program

Keynote Session:

Meetings International -  Conference Keynote Speaker Mark Woodward photo

Mark Woodward

University of Oxford, UK

Title: Cardiovascular Disease, Classical Risk Factors and Nutrition: The Role of Sex Differences


Mark Woodward PhD F Med Science works at the George Institute for Global Health. He is Professor of Statistics and Epidemiology at the University of Oxford, UK, Professor of Medical Statistics at the University of New South Wales, Australia and Adjunct Professor of Epidemiology at Johns Hopkins University, USA. His main research areas are cardiovascular disease, renal disease and diabetes. His recent work has had a particular focus on sex differences, in which he has a leadership role at the George Institute. He has published two text-books on medical statistics and well over 600 peer-reviewed publications. In each of the last five years, Professor Woodward was named by Thomson Reuters/Clarivate Analytics as one of ‘The World’s Most Influential Scientific Minds’.


Statement of the Problem:

Cardiovascular disease is the under-recognized primary cause of death and disability in women worldwide, both in high- and low-income populations. One of its major risk factors is obesity, which is a major factor in the development of diabetes. Evidence suggests that obesity is increasing more in women than men. In turn, diabetes is itself a leading risk factor for CVD, with a greater effect on women than men. Nutrition is a basic issue in this chain of causality, and hence sex differences in nutrition are of fundamental importance. Methodology and Theoretical Orientation: Meta-analyses were used to identify sex differences in the obesity-diabetes-CVD triad. Cross-sectional data on over 200,000 people (52% women) in the UK Bio bank were used to explore sex differences in macronutrient intakes and adherence to dietary recommendations. Findings: Men had greater intakes of energy but were less likely to have energy intakes above the estimated average requirement compared with women. For all macronutrients, men had greater absolute intakes while women had greater intakes as a percentage of energy. Women were more likely to have intakes that exceeded recommendations for total fat, saturated fat and total sugar. Men were less likely to achieve the minimum recommended intakes for protein, polyunsaturated fat and total carbohydrate. Sex differences in dietary intakes were moderated by age and, to some extent, by socioeconomic status. Conclusion & Significance: There are significant sex differences in adherence to dietary recommendations, particularly for sugar. Dietary interventions and advice may need a specific focus on women, as part of a recommended life-course approach to women’s health (Bennett et al, BMJ Open, 2018).

Meetings International -  Conference Keynote Speaker Jerome Foucaud photo

Jerome Foucaud

National Institute of cancer, France

Title: Awareness of Nutritional Risk Factors for Cancer among the French Population: The French Cancer Barometer


Jerome Foucaud holds a PhD in Humanities sciences. He is the Head of the department for research in Humanities and Social Sciences, Epidemiology and Public Health at the French National Cancer Institute. In this National Health and Scientific Agency for cancer control, he is charge to build up research and to lead the elaboration of data evidence about cancer in Humanities and Social Sciences. He is also associated researcher to the Health Education and Practices Laboratory (LEPS EA 3412). In parallel of these activities, he teaches patient education in different universities in France.


Statement of the Problem:

Different factors are known to increase or decrease cancer risk (e.g., tobacco use, alcohol, diet). To date, few national studies have been conducted to investigate individuals’ perceptions, attitudes and behaviors linked to these factors to adapt national prevention strategies and to raise public awareness on cancer. In this line, the French Cancer Barometer, a population-based-survey, is carried out every five years. However, individuals’ perceptions on nutritional risk factors have been little studied to date. Hence, our aims are: (1) to identify the least perceived nutritional risk factors for cancer, and (2) to assess the evolution of the French population perceptions on cancer risks over time (2010-2015).


A randomly selected sample of participants aged 15-75 years (n=3345 in 2010) and 15-85 years (n=3764 in 2015), representative of the French population, were interviewed. Questions on perception of diet, physical activity, obesity and breastfeeding as cancer risk factors were asked. Findings: In 2015, nutritional factors were well perceived by the participants as having an important role in cancer development: diet (90.8%), physical activity (70%), obesity (76%); except for breastfeeding (34%). Some diet factors were also moderately perceived, such as the benefits of fruits and vegetables (58.1%) or the risks of red meat (43%) and salt and salted foods (55%) consumption. Age and education were associated to the perception of nutritional risk factors. In 2015, compared to 2010, nutritional risk factors were more perceived, and participants had stronger opinion.

Conclusion & Significance:

Nutrition is perceived by the French population in 2010 and 2015 as an important factor in cancer development, but some factors are still not very well perceived in 2015.Demographic variables associated to a lesser perception are highlighted. Thus, recommendations to improve prevention strategies and to raise public awareness are made.

Meetings International -  Conference Keynote Speaker Shahryar Eghtesadi photo

Shahryar Eghtesadi

Azad University School of Medical Sciences and research,Iran

Title: Study of the Association of the Dietary Pattern and Chronic Diseases Effective Factors on the General Health Condition of Tehrans Opposite Gender Adult Twins


Dr. Shahryar Eghtesadi received Bachelor degree in Nutrition Science and Food Chemistry 1975, from Shahid Beheshti University of Medical Sciences, Tehran; MSPH degree in Nutrition, 1977, from Tehran University of Medical Sciences, Tehran and PhD from University of  California at Davis(UCD), USA, in Nutrition (1985). He  served as Visiting Scientist in USDA Human Nutrition Research Center on Aging (HNRCA),Tufts University, Boston, USA (1994-1995); Full professor of Tabriz, Iran and Tehran Universities of Medical Sciences and currently serves  as Professor of Azad University, Science & Research Branch,Tehran . He  was the chairs of Departments of Nutrition and Biochemistry, Biochemistry & Clinical Nutrition, Public Health Nutrition and Nutrition  in aforementioned Universities. Also Served as Associate Dean of  School of Public Health & Nutrition and Dean of School of Public Health of Tabriz and Iran Universities of Medical Sciences respectively.He was selected as distinguished professor and Scientist, for long and extended period of time, experienced  teaching various courses in nutrition in undergraduate, graduate and postgraduate and  international Bureau  programs and directed  many projects and dissertation of MS and PhD programs and Published numerous peer reviewed articles in journals and also edited several books and finally served as Principal Investigator of World Bank Project for Capacity Building in Nutrition in Iran.


Statement of problem:
Few studies have examined dietary patterns among Twins and as far as we know, no study has been carried out in Iran by far. The aim of this study was to determine the relationship between dietary patterns and chronic disease’s effective factors on the general health condition of Tehran’s Opposite Gender Adult Twins.
Methodology&Theroretical Orientation :
A group of 132(66 pair) Opposite Gender Twins participated in this cross-sectional study. For all participants Semi-quantitative food frequency, International Physical Activity, General Health and general questionnaires were completed and their anthropometric characteristics, body composition and blood Pressure were determined. Factor analysis was used to identify major dietary patterns. Association between general health with dietary patterns and chronic disease effective factors were estimated using logistic regression analysis.
Findings: Two major dietary patterns(western & healthy) were identified. Significant differences between first and second Twins were observed in physical activity, body mass index and intakes of glucose, fructose and maltose. The results showed that after adjustment for confounding factors, in first Twins,only birth weight and physical activity, whereas , in second Twins besides birth weight, gender, marital state and body fat percentage were among the most significant factors in determining general health of participants.
Twins with low birth weight significantly were likely to have more health problems. Identified food pattern has no association  with general health in Twins, however further studies are recommended and warranted.
Meetings International -  Conference Keynote Speaker Rose Marie Boylan photo

Rose Marie Boylan

Registered Dietitian, Canada

Title: Sexual Assault, Body Identity & Disordered Eating: Food & Mind-Body Medicine for Improving Health Outcomes


Rose-Marie Boylan, BSc. M.A. is a Dietitian by trade. She has worked across 6 of the largest pharmaceutical companies for over 27 years, and 4 smaller organizations. She has specialized in patient access to medicines, patient reported outcomes, health & economics, policy influence for patient self-sovereignty. In parallel, Rose-Marie has researched for over 13 years social, civil and political cost-consequence & cost-benefits of psychiatry, transpersonal psychology & human flourishing in violence survivors. This includes a cost-consequence model. She completed her Masters in 2009 in Leadership Studies with research on the optimum levels that humans can attain in a life time for developing the highest levels of moral, emotional & cognitive intelligence through self-actualization & post-traumatic growth. Leveraging the research of Abraham Maslow & Jane Loevinger on ego development she developed a model which defines mind health vs. psychopathologies. Additionally for 13 years Rose-Marie studied mind-body medicine with the likes of Dr. Herbert Benson from Harvard’s Mind Body Institute, Jon Kabat- Zin, Saki Santorelli from the University of Massachusetts School of Preventive & Behavioral Medicine. Additionally researching all forms of meditation, therapeutic & mindfulness yoga teacher trainings which she has taught to trauma survivors & professionals in crisis.



Problem Identification:

According to RAINN.org (2017), “every 98 seconds an American is sexually assaulted. When broken down one in 4 girls and one in six boys before the age of 18 will experience some form of sexual abuse. One in five women and one in 16 men are sexually assaulted in college.” (National Sexual Violence Resource Center,  2015). “Sexual assault is underreported, with only 63% of cases reported to authorities and just 12% of child sexual abuse cases.”(NSVRC, 2015) How the female psyche responds to sexual assault and boundary violations is different than males.

Clinical decision-making:

Following sexual assault it is expected that the subject will experience trauma-related symptoms. “In fact, up to 94% of women who are raped have experience post-traumatic stress disorder.” (Barbash, E. 2017) Up to “70% of sexual assault survivors experience moderate to severe distress, larger than any other violent crime according to the National Sexual Violence Resource Center, 2015.” Females who experience sexual assault and/or violence may have a predisposition to self-harm vs. harming others.

Self-harm can range in addictive, high-risk or other harmful behaviors. Following the experience of trauma theremay be underlying neurobiological mechanisms at play which can lead to disordered eating. Disordered eating may include binge eating, food addictions, Hedonic eating, bulimia and anorexia nervosa.

In this research we introduce how trauma-related events when treated with healthy & empowering interventions can improve recovery and lead to better health outcomes. Trauma when treated with positive psychology principles may improve mind health & ego development in humans when addressed appropriately within a framework of creating post-traumatic growth. The research has been done over 13 years including a Master’s in leadership Studies reviewing the most developed brain systems cognitively, emotionally & morally for social progress & human flourishing. We have evaluated historical practices from the Vedic Sciences, psychiatry,nutritional sciences to develop a model for improving health economic and patient-reported outcomes following sexual assault. The model includes mindful eating, mindfulness meditation and food as medicine for grounding the subject after trauma. These techniques may promote better health outcomes for the nervous system reducing subject related reactions to memories.

Stress arousal has an impact on arousing the sympathetic nervous system response. Other interventions including certain nutritious foods can disengage stress arousal stimulating the parasympathetic nervous system response for grounding and calming the survivor. We discuss the role sympathetic nervous system response has in producing “fight, flight or freeze” responses upon arousal. We further elaborate on historical practices from the Vedic sciences, nutrition and the role they can play with mind-body medicine at accelerating recovery.


Oral Session 1:

  • Oral Session -1
Meetings International -  Conference Keynote Speaker Ghada Ahmed El hag Mohamed photo

Ghada Ahmed El hag Mohamed

King Abdulaziz University, Saudi Arabia.

Title: Assessment of Fish Oil Effects on the Testicular Structure of Male Rats


Ghada Ahmed El hag Mohamed is a Associate Professor of Zoology in King Abdulaziz University, Jeddah, Saudi Arabia.


The aim of this study was to investigate the protective effects of fish oil on lead acetate induced testis toxicity rats. Forty male rats were randomly divided into 4 groups, 10 rats in each. Control rats received normal saline, while treated rats received lead acetate (150 mg/kg body weight) three times weekly, lead acetate and fish oil (650 mg/kg body weight/day) and fish oil (650 mg/kg body weight/day) for six weeks by gavage tube.

The results showed an insignificant increase in mean testis weight with highest and lowest testis weight gained was of 3.09±0.31g and 2.82±0.76 g respectively. Testicular histology of rats treated with fish oil revealed slight changes in the uniformity of arrangements of seminiferous tubules.

Data from present study suggests that fish oil have been initiating the positive effects on testicular normal structure in lead acetate treated rats.


Meetings International -  Conference Keynote Speaker Wuyeh Drammeh photo

Wuyeh Drammeh

Univerity of Sains, Malaysia

Title: Household Food Insecurity and its Determinants in Lower River Region, Gambia


Mr. Wuyeh Drammeh is a student in department of Community Medicine, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia.


Food insecurity is a major challenge for rural households in the Central River Region. However, little is known about its determinant’s factors. The purpose of this study was to investigate the factors affecting household food security. 
A cross-sectional study was conducted among 334 households who have been recruited through multistage random sampling in the Central River Region. Women, as a proxy to the household, consented for the study were then interviewed the Household Food Insecurity Access Scale (HFIAS) questionnaire. Data were analyzed using SPSS version 24 and multiple logistic regression was conducted.  
334 households were included in this study.  Three-quarters of the households (73.4%) were food insecure% households mildly food insecure, 14.1% moderately food insecure and 50.3% severely food insecure. Female household headed  (AOR = 4.8, 95% CI:1.01, 5.84),  household size of ≥7 members  (AOR = 4.5,95% CI:1.18, 5.92), household  income (AOR = 1 4.8,95% CI:3.74, 15.67), absence of livestock (AOR = 3.42, 95% CI:1.21, 4.85), women’s occupation  (AOR = 10.5, 95%CI:1.31, 14.47),  spouse’s occupation (AOR = 4.55, 95% CI:2.08, 9.75), lack of financial assistance  (AOR = 2.42, 95% CI:1.03,5.67) and lack of market access to food  (AOR = 2.2, 95%CI:1.18, 4.10), were factors associated with  household food insecurity. 
This current study found that there was a high prevalence of household food insecurity in Central River Region, Gambia. Future studies are needed to provide evidence in another region of Gambia in order to generate policy so that measures are taken against this food insecurity problem.
Meetings International -  Conference Keynote Speaker Md.Nazmul Huda  photo

Md.Nazmul Huda

Government Unani & Ayurvedic Medical College, Bangladesh.

Title: The Role of Ayurveda for the Prevention of Non Communicable Diseases


Md. Nazmul Huda is a lecturer in Department of Ayurvedic Medicine of Government Unani & Ayurvedic Medical College, Dhaka in Bangladesh.


Non communicable diseases (NCDs) are the major cause of morbidity and mortality in worldwide. By 2020, it is predicted that these diseases will be causing for 73% of deaths globally among them 60% of the disease burden will be in the developing countries. In Bangladesh, most recent study suggests that NCDs are responsible 68% of total mortality while communicable diseases account for only 11% of total deaths. Therefore this is a great challenge to minimize the burden of NCDs in Bangladesh. Moreover, currently there is no routine surveillance of NCD’s related risk factors or morbidity and mortality and no subsidized treatment for non-communicable diseases through the public health system in Bangladesh. So this study attempted to determine the awareness of risk factors NCD’s through the modification of life style by the knowledge of Ayurveda. Ayurveda is known as leading life science and describes ways to prevent the risk factors of NCD’s and manage lifestyle disorders in  the  forms  of  proper  dietary  management,  the advice of daily and seasonal regimen,  some detoxification and bio-purification procedures, mediations, and rejuvenation therapies. 
Qualitative methods including various  Ayurvedic  classic  texts and scientific journals  have  been  used  for  this  study  as  source materials. 
The burden of NCD’s is affecting countries worldwide but with a growing trend in developing countries and a special attention is devoted to avoiding the risk factors of NCD’s through the knowledge of Ayurveda. 
This  conceptual  study  emphasize  the  importance  of  the  Ayurvedic  principles  and  implementing in the day to day life to keep away from the various NCD’s.  
Meetings International -  Conference Keynote Speaker Ha T Nguyen  photo

Ha T Nguyen

Medical doctor, Vietnam

Title: Nutritional Status and Quality of Life in Vietnamese Outpatients with Chronic Obstructive Pulmonary Disease (COPD)


Ha Nguyen has her expertise in evaluation and passion in improving healthy life style, especially in promoting healthy eating habits. Her open and contextual intervention model based on “4A principles” – Available, Accessible, Affordable, and Acceptable - which creates effective pathways for improving healthcare. She has studied and applied this model after years of experience in research, evaluation, teaching and administration both in hospital and education institutions. Currently she acts as the Director of the Department of Nutrition and Dietetics, National Lung Hospital, Vietnam and a senior lecturer of the Nutrition and Food Safety Department of the Hanoi Medical University, Vietnam. She is a member of the Nutrition for Lung Transplant Technical Advisor Groups of Australia, New Zealand, Canada and Vietnam. She is also an active member of the Vietnam Nutrition and Dietetics Association. Her research is focused on nutrition for people with lung diseases in both hospitals and communities. 


Statement of the Problem:
Malnutrition is common in patients with chronic obstructive pulmonary disease (COPD), however little is known about its impacts on health-related quality of life among patients with COPD.  This study aimed to explore the nutritional status and dietary intake among outpatients with COPD in Vietnam and its possible associations with quality of life (QoL).
A cross-sectional study was carried out in COPD outpatients visiting the COPD management unit at the National Lung Hospital, Hanoi, Vietnam between May 2017 and July 2017. Consecutive outpatients with a confirmed diagnosis of COPD were recruited with written inform consent. The nutritional status of participants was assessed using Subjective Global Assessment (SGA), and dietary intake via a 24-hour recall interview. The St George Respiratory Questionnaire for COPD was used to investigate the participants’ QoL.  Socio-demographic and clinical data was extracted from hospital records. Findings: Of 168 COPD outpatients involved in the study, three-quarters (74.4%) were diagnosed as malnourished (SGA B/C) and 81.5% reported unintentional weight loss. Most did not meet their estimated energy and protein requirements (85.7% and 89.9%, accordingly). Malnutrition was significantly associated with disease severity (p=0.039), and ratio of protein intake to estimated requirement (%EPR) (p=0.005). QoL was low for all levels of malnutrition or disease severity, with well-nourished participants and those with less disease severity having better QoL (p=0.006 and p<0.001, accordingly). With an extra meal per day, the odds of having malnutrition decreased 5.6 times (p<0.05) and the total SGRQ reduced 3.61 scores (p<0.05) indicating a better QoL.
Conclusion & Significance:
Malnutrition and weight loss is prevalent among COPD outpatients. Most had inadequate dietary intake and low QoL. Nutrition counselling on increasing the number of meals per day with a focus on energy- and protein-rich foods may help improving nutritional status and QoL of patients with COPD in Vietnam


Meetings International -  Conference Keynote Speaker Sahar Talebi photo

Sahar Talebi

Isfahan University of Medical Sciences, Iran

Title: Genotoxic Effect of Sodium Fluoride on Stem Cells from Human Exfoliated Deciduous Teeth


Sahar Talebi  has graduated in dentistry. She is working as a dentist and clinician, her field of interest is tooth development and dental stem cell. She is experienced in isolation, culture and preservation of stem cell from human exfoliated deciduous teeth.


Due to its unique properties, Fluoride has been widely used for the prevention and control of dental caries in dentistry. However, there is much controversy over its toxicity effects such as dental fluorosis, skeletal fluorosis, reproductive, renal, gastrointestinal, immunological, neurological, genotoxicity and carcinogenicity. As DNA damage is the primary step in the carcinogenesis process, so the aim of this study was to evaluate of fluoride genotoxicity on Stem cells from Human Exfoliated Deciduous teeth (SHED). Stem cells are the target of carcinogenesis.
Materials and Methods:
SHED in culture medium were exposed directly to sodium fluoride (NaF) at concentrations of 10, 50, 100 PPM. We used methalmetasulfonate(MMS)at 1,5, and 15μg/ml as the positive control and the negative control was culture medium for mesenchymal stem cells. The comet assay was carried out for detecting DNA damage. Damaged cells are comet-like under fluorescence microscopy. Comet analysis was accomplished using computerized image analysis software (CASP). Four parameters containing %Tail DNA (%TD), Tail Length(TL), Tail Moment (TM) and Olive Tail Moment(OTM) were evaluated. Data were analyzed with SPSS version 15.
Results: Kruskal-Wallis test show that the mean of four parameters was significantly in all the groups (p<0.05).
NaF at different concentrations was able to induce DNA damage in SHED.
Meetings International -  Conference Keynote Speaker Said Poyan Sadat photo

Said Poyan Sadat

Ariana University School of Medicine, Afghanistan.

Title: Encyclopedia of Bioavailability and Bioeqivalence- Nutrition and Its Importance


Dr. Said poyan sadat has completed his MD at the age of 25 years from Ariana University of Medical . He is working as a HMIS Manager in Afghan Red Crescent Society. And also he has working as a medical doctor in a Hospital, part time 


Introduction :

Nutrition, nourishment, or aliment, is the supply of materials - food - required by organisms and cells to stay alive. In science and human medicine, nutrition is the science or practice of consuming and utilizing foods.In hospitals, nutrition may refer to the food requirements of patients, including nutritional solutions delivered via an IV (intravenous) or IG (intra gastric) tube. Nutritional science studies how the body breaks food down (catabolism) and how it repairs and creates cells and tissue (anabolism). Catabolism and anabolism combined can also be referred to as metabolism. Nutritional science also examines how the body responds to food.                           

 Fast facts on nutrition: 

  • The human body requires seven major types of nutrients.
  • Not all nutrients provide energy but are still important, such as water and fiber.
  • Micronutrients are important but required in smaller amounts.
  • Vitamins are essential organic compounds that the human body cannot synthesize.


Meetings International -  Conference Keynote Speaker Marjan Farzad  photo

Marjan Farzad

Birjand University of Medical Sciences, Iran

Title: Impact of lifestyle training on serum lipids of children and adolescents with dyslipidemia: a quasi-experimental study


Dr.Marjan Farzad is a research assistant at cardiovascular diseases research center and also a faculty member of Birjand University of Medical Sciences in Iran .



Discussion :

Dyslipidemia is one of the most important risk factors for cardiovascular diseases. It can cause a rise in the incidence of these diseases in adulthood. Lifestyle modification is a determinant factor for incidence,prognosis and complications of cardiovascular diseases.The aim of the study was to evaluate the effect of lifestyle modification on serum lipids of children and adolescents with dyslipidemia.This quasi-experimental study was conducted on all dyslipidemic children and adolescents referred to the cardiovascular diseases research center of Birjand University of Medical Sciences, Iran. Participants underwent 6 sessions of training, appropriate to their age, including nutrition and  healthy lifestyle. Before the study and 6 months after trainings, in the same circumstances, subjects were examined for level of blood lipids and blood pressure. Data were analyzed using paired-samples t- test, Pearson correlation coefficient, and independent-samples t-test. P-value of less than 0.05 was considered statistically significant.Of 50 children with dyslipidemia, 52% were female and 48% were male. The mean age of participants was 12.77±1.84 years. The study showed a significant difference between the mean of cholesterol (p<0.001) and LDL (p=0.008) before and after the study. Comparing the changes made in the mean of variables such as FBS, Cholesterol, TG, HDL and LDL as a result of gender-based lifestyle modification, no significant difference between the two sexes was seen (p>0.05). Pearson correlation coefficient showed no significant relationship between the mean of these variables in children as a result of lifestyle modification and their age (p>0.05). Lifestyle modification relying on exercise and physical activity, nutrition and sufficient sleep and rest can be effective as a non-pharmacological supplement in reducing blood lipids and dyslipidemia. Lifestyle modification training is essential for both children and their parents after diagnosis of dyslipidemia as a part of the therapeutic plan.

Meetings International -  Conference Keynote Speaker Batool Sadeghi Nejad  photo

Batool Sadeghi Nejad

Abadan School of Medical Sciences, Iran.

Title: Antimicrobial Efficacy of Polyherbal -made Toothpaste and Mouthwash against Oral Pathogens: A Randomized Clinical Trial


  1. Batool Sadeghi-Nejad Graduated of bachelor degree (B. Sc.) in the field of public health from Isfahan University of Medical Sciences on Feb. 15 1988
  2. Holder of master degree (M. Sc.) in the field of Medical Parasitology from Isfahan University of Medical Sciences on April 30 1997.
  3. Holder Ph.D. degree in Medical Mycology from Botany department of Pune University, India on Feb. 2008..  She is members of Scientist Academic Institution, Abadan School of Medical Sciences, Abadan, Iran (Assistant professor, Ph.D Medical Mycology). She has published more than 24 papers in reputed journals and has been serving as an reviewer board member of repute.          


Background: The aim of this study was to evaluation in vivo antimicrobial effects of polyherbal-made toothpaste and mouthwash to form effervescent tablet containing the extracts of Artemisia dracunculus, Satureja khuzestanica and Myrtus communis against oral pathogenic microorganisms (Streptococcus sanguinis, Streptococcu salivarious, Streptococcu mutans and Candida albicans).

Methods: This study was clinical trial without control (Before – After test) that were performed on 20 volunteers, healthy with normal gums without bleeding or inflammation. Twenty volunteers will be given herbal toothpaste and asking from them to wash their mouths by sterile deionized water, pour the contents of their mouth into sterile container as samples of before test. Then asked them to use brushing their teeth with herbal toothpaste. The next morning again asked from same volunteers to wash their mouths by deionized water for one minute and pour the contents of their mouth into another sterile container as sample of after test. Then the samples transferred to laboratory immediately. Aliquots of 100µl of each sample inoculated on petri dishes containing blood agar for bacteria and Sabouraud dextrose agar for Candida albicans. Then colonies counted before and after test, separately. This process were repeated for the herbal effervescent tablets mouthwash by other 20 volunteers with same condition.


According to the laboratory results, the Candida albicans was isolated from all the voluntaries. The scores of colony growth decreased significantly between before - after test during the studies (p<0.001).                                                                                                      

Conclusion: The results were revealed that polyherbal toothpaste and mouthwash could be effective for prevention of prevention of dental caries in future.