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Stem Cell

May 29, 2021

Scientific Program

Keynote Session:

Meetings International -  Conference Keynote Speaker Peter Everts photo

Peter Everts

Gulf Coast Biologics, USA

Title: Assessing clinical implications and perspectives of the pathophysiological effects of erythrocytes and plasma free hemoglobin in autologous biologics for use in musculoskeletal regenerative medicine therapies

Biography:

Peter Everts has been pioneering in the science and clinical applications of platelet-rich plasma  technology since 1984 and has been leading research teams in regenerative medicine in using biological therapies.Everts received his Ph.D in Medicine from the University of Utrecht in the Netherlands in 2007 on the subject of PRP. At present, he has published and authored more than 50 articles, or book chapters. He has been board member, and chairman of several health care organizations and was member of the sport medicine steering group at the International Olympic Committee. At present he is the executive director of Gulf Coast Biologics, a scientific-educational-research-training institute, and chief scientific Officer of  EmCyte corporation.

Abstract:

Autologous biologics, defined as platelet-rich plasma and bone marrow aspirate stem cell concentrate are cell-based therapy treatment options in regenerative medicine practices, and have been increasingly used in orthopedics, sports medicine, and spinal disorders. These biological products are produced at point-of-care; thereby, avoiding expensive and cumbersome culturing and expansion techniques. Numerous commercial PRP and BMSC systems are available but reports and knowledge of bio-cellular formulations produced by these systems are limited. This limited information hinders evaluating clinical and research outcomes and thus making conclusions about their biological effectiveness. Some of their important cellular and protein properties have not been characterized, which is critical for understanding the mechanisms of actions involved in tissue regenerative processes. The presence and role of red blood cells  in any biologic has not been addressed extensively. Furthermore, some of the pathophysiological effects and phenomena related to RBCs have not been studied. A lack of a complete understanding of all of the biological components and their functional consequences hampers the development of clinical standards for any biological preparation.This lecture aims to review the clinical implications and pathophysiological effects of RBCs in PRP and BMSC; emphasizes hemolysis, eryptosis, and the release of macrophage inhibitory factor; and explains several effects on the microenvironment, such as inflammation, oxidative stress, vasoconstriction, and impaired cell metabolism.

Meetings International -  Conference Keynote Speaker Masahiro Onuma photo

Masahiro Onuma

Trisguid Ltd, Japan

Title: Electronic water can reduce oxidative stress in cancer and diabetes patients for 3 weeks drinking

Biography:

Masahiro Onuma has expertise in oxidative disease prevention to use non-medical product based on GSK’s experience of Allopurinol which is the strongest anti-oxidant efficacy in this world. He creates new indication of allopurinol for stomatitis induced by cancer treatment which was approved by the Japanese cancer treatment committee to propose new mechanism of allopurinol for anti-oxidant. And now, there are so many new research papers of allopurinol in the world.

Abstract:

Oxidative stress means a state there is imbalance between the oxidizing action and the reducing action due to reactive oxygen species (ROS) in a living body, resulting in the oxidizing action becoming dominant.  Oxidative stress arises as the balance between production and removal is disrupted through excessive production of ROS and impairment of the antioxidant system.  Oxidative stress has been reported to be involved in the onset and progress of various diseases.  Characteristics of Type 2 diabetes are insulin secretion failure and insulin resistance, but it seems that oxidative stress is greatly involved in insulin secretion failure.  In the insulin secretion-inducing β cells of Langerhans islets in the pancreas, the amount of superoxide dismutase (SOD), which is representative of the ROS elimination system, is small and resistance to oxidative stress is considered to be weak. Regarding cancer, it is well known that chronic inflammatory conditions increase the risk of carcinogenesis.  Cells such as neutrophils and macrophages are activated in the inflammation area leading to increase in production of active oxygen and nitric oxide.  These free radicals cause DNA mutation and cell proliferation thereby promoting cancer development.  When chronic inflammation is present, cancer develops more easily. 
 
Electronic water, which was developed to generate electron in water, was consumed for three weeks, after meals, between meals and before sleeping 6 times a day, and according to the test subjects' possible time periods.  The amount of drinking water was 750-1000 mL, and BAP and d-ROMs checks for all cases were carried out at 4:30 pm.  The results of cancer patients and diabetes patients were seen as attached.
 
Oxidative stress means a state there is imbalance between the oxidizing action and the reducing action due to reactive oxygen species (ROS) in a living body, resulting in the oxidizing action becoming dominant.  Oxidative stress arises as the balance between production and removal is disrupted through excessive production of ROS and impairment of the antioxidant system.  Oxidative stress has been reported to be involved in the onset and 
Meetings International -  Conference Keynote Speaker Talha Bin Emran photo

Talha Bin Emran

BGC Trust University, Bangladesh

Title: Baculovirus as a new stand-alone prophylactic and therapeutic immunostimulatory agent against malaria

Biography:

Talha Bin Emran has been working as an assistant professor in the Department of Pharmacy at the BGC Trust University Bangladesh. Dr. Emran has had his B.Sc. honors and M.S. in Biochemistry & Molecular Biology from the University of Chittagong, Ph.D from Graduate School of Medical Sciences, Kanazawa University, Japan. 

Abstract:

Baculovirus which is an enveloped insect virus with a circular double-stranded DNA genome, possesses unique characteristics to induce strong innate immune responses in various mammalian cells and in mice. Aim: Here we show that the innate immune responses induced by BV not only eliminate Plasmodium liver-stage parasites but also elicit sterile protection against Plasmodium sporozoite infection through type I IFN signaling pathway. Methodology: Mice had infected with liver-stage parasites before 24h completely prevented blood-stage parasites following a single dose of BV intramuscular (i.m.) administration, which was much superior to primaquine, the only drug approved to eradicate liver-stage parasites. Findings: This BVmediated liver-stage parasite elimination was also observed in TLR-9-/- and iNOS-/- mice. In addition to the therapeutic effect, BV i.m. administration sterilely protects mice for at least 7 days from subsequence sporozoite infection, indicating the prophylactic effect. In vivo passive transfer with sera from mice i.m. administered with BV effectively eliminated liver-stage parasites and this effect was canceled by neutralization of IFN-a but not IFN-g in the sera, indicating a killing mechanism downstream of type I IFN signaling pathway. In fact, 6h after BV i.m. administration, both type I and II IFNs were robustly produced in sera and RNA transcripts of interferon-stimulated genes were drastically upregulated in the liver. Conclusion & Significance: Our results provide a great potential of BV for development of BV-based vaccine and anti-hypnozoite drug as a new stand-alone therapeutic and prophylactic immunostimulatory agent, which is applicable not only for malaria but also for other serious infectious diseases such as viral hepatitis.

Meetings International -  Conference Keynote Speaker Baljeet Singh photo

Baljeet Singh

Lovely Professional University, India

Title: The battle against different fungal diseases in wheat by both conventional and modern approaches

Biography:

He is a Ph.D student  in Lovely Professional University,India

Abstract:

WHeat ranked 2nd in terms of global production and opted as primary staple food by many countries across the world. With the advent of technology and improved varieties the balance between wheat grain supply and demand was maintained in past few decades. However, rapidly increasing population and changing food habits are widening the gap between supply and demand. There is an urgent need of considerable increase in wheat production to fulfil the global food requirement. Many biotic and abiotic stresses diminishing the plant growth and grain yield. Among all biotic stresses, fungal diseases are most dangerous and widely spread throughout the planet. Fungal disease eradication and management with the help of both traditional and modern approaches can significantly increase the grain yield, as each year there is about 15-20% yield loss majorly due to rusts, blotches, smuts, blights and scabs. For the development of fungal disease resistant, varieties traditional breeding methods seems not as promising as they are labours and time consuming. However, increasing genomic information regarding wheat provided the platform for various genome editing and reverse genetic approaches. In this review, we described the impact of different fugal diseases and also discussed their management strategies.